Comparative study of antioxidant, metal chelating and antiglycation activities of Momordica charantia flesh and pulp fractions
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 4
Abstract
Momordica charantia is commonly used as a vegetable and folk medicine in most parts of South Asia. This study aims to determine and compare the antioxidant, metal chelating and antiglycation activities of aqueous extracts of M. charantia fruit flesh (MCF) and fruit pulp (MCP) fractions. Our results show that MCP has pronounced DPPH and ABTS radical scavenging potential compared to MCF. In the antiglycation assay both fractions illustrated considerable inhibitory activities against the formation of AGEs induced by glucose with an efficacy of 75 and 67% with 150 µl of MCP and MCF extracts respectively, almost equal to 0.3mM amino guanidine. Results for metal catalysed protein fragmentation and autoxidative and glycoxidation assays demonstrate that MCF and MCP inhibited metal catalysed protein fragmentation. The percentage of relative standard deviation for three replicate measurements of 150 µl of MCF and MCP was ≤ 3.0% for antiglycation. The antioxidant assays with regression values of MCP (0.981 and 0.991) and MCF (0.967 and 0.999) were also recorded. We conclude that both extracts possess high antioxidant and antiglycation activities and are equally good sources of antioxidant and antiglycating agents.
Authors and Affiliations
Tahseen Ghous , Nouman Aziz , Zahid Mehmood , Saiqa Andleeb
Keywords
Related Articles
- EP ID EP111533
- DOI -
- Views 79
- Downloads 0
Green approach towards the determination of hydroxyzine dihydrochloride in pure and pharmaceutical dosage forms
A simple eco-friendly method has been developed for detection of hydroxyzine dihydrochloride in pure and pharmaceutical dosage forms. Both conventional system and microwave assisted procedures are used for the deve...
A comparison among three different analytical methods to test the scavenging properties of different integrators against radicalic stress
The aim of the present work was to experimentally evaluate the total antioxidant capacity of different commercially available pharmaceutical integrators based on vitamin E, astaxanthin, resveratrol and blueberry us...
Evaluation of antioxidant potential and HPLC based identification of phenolics in Polygonum amplexicaule extract and its fractions
There is a growing interest for the plant-based medicines in pharmaceutical industry. Plant derived Antioxidants have gained huge importance regarding their medicinal value. The present study was designed to establ...
Anti-nociceptive activity of seed extract of Vernonia anthelmintica willd
Vernonia anthelmintica is commonly known as kali ziri. Its seeds are used for several therapeutical purposes. Its seeds contain many constituents of medicinal importance as vernodlin, vernodalol, and vernolic acid....
Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra
Recent worldwide inclination for the consumption of natural compounds has extremely augmented the significance of persistent quality of plant materials. Consequently, there is an escalating scientific concern in th...