Comparison of drug susceptibility pattern of Mycobacterium tuberculosis assayed by MODS (Microscopic-observation drug-susceptibility) with that of PM (proportion method) from clinical isolates of North East India
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 2
Abstract
About one third of the world’s population are infected with tuberculosis and has become a serious global concern of public health. The major problem in controlling tuberculosis is the rapidity and efficacy of detection methods adopted. This study was conducted with the objective to compare the drug susceptibility pattern of Mycobacterium tuberculosis assayed by MODS (Microscopic-observation drug-susceptibility) with that of PM (proportion method) from clinical isolates of North East India. A total of 150 smear positive sputum specimens clinical isolates of M. tuberculosis referred to a clinical lab wa selected. Both the proportion method and MODS were conducted for the collected isolates. DST of MODS were compared with that of PM considering PM as gold standard. MODS detected INH, RIH, STR and EMB resistant isolates at 31% (n = 46/150), 29% (n = 49/150), 19.3& (n = 29/150) and 27.3% (n = 41/150), respectively .Specificity was very high for all the drugs resistance with more than 99% specificity. The accuracy, PPV and NPV of MODS in detection of the four drug resistant isolates was relatively high. DST assay by MODS is relatively simpler. The appreciable performance characteristic in detecting drug resistance including MDR TB may lead to its wider applications in different labs and general hospital for resource limited regions.
Authors and Affiliations
Leimapokpam Shivadutta Singh , Pranab Behari Mazumder , Gauri Dutt Sharma
Keywords
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