Comparison of drug susceptibility pattern of Mycobacterium tuberculosis assayed by MODS (Microscopic-observation drug-susceptibility) with that of PM (proportion method) from clinical isolates of North East India
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 2
Abstract
About one third of the world’s population are infected with tuberculosis and has become a serious global concern of public health. The major problem in controlling tuberculosis is the rapidity and efficacy of detection methods adopted. This study was conducted with the objective to compare the drug susceptibility pattern of Mycobacterium tuberculosis assayed by MODS (Microscopic-observation drug-susceptibility) with that of PM (proportion method) from clinical isolates of North East India. A total of 150 smear positive sputum specimens clinical isolates of M. tuberculosis referred to a clinical lab wa selected. Both the proportion method and MODS were conducted for the collected isolates. DST of MODS were compared with that of PM considering PM as gold standard. MODS detected INH, RIH, STR and EMB resistant isolates at 31% (n = 46/150), 29% (n = 49/150), 19.3& (n = 29/150) and 27.3% (n = 41/150), respectively .Specificity was very high for all the drugs resistance with more than 99% specificity. The accuracy, PPV and NPV of MODS in detection of the four drug resistant isolates was relatively high. DST assay by MODS is relatively simpler. The appreciable performance characteristic in detecting drug resistance including MDR TB may lead to its wider applications in different labs and general hospital for resource limited regions.
Authors and Affiliations
Leimapokpam Shivadutta Singh , Pranab Behari Mazumder , Gauri Dutt Sharma
Keywords
Related Articles
- EP ID EP141713
- DOI -
- Views 73
- Downloads 0
The Development Extract Protein of Bambara Nut (Vigna subterranea (L.) Verdc.) As a Reagent for Detecting Food Allergies on Skin Prick Test Method
This study aimed to prepare the protein extract of bambara nut seeds, characterize and apply as a reagent for Skin Prick Test (SPT). Bambara nut protein was extracted from seeds flour of Bambara nut by isoele...
Evalution of Anti Microbial Activity and Phytochemical analysis of different organic solvent of Calotropis gigantea
The leaf and fruits extract of Calotropis gigantea were screened for its anti microbial and phytochemical activities. The solvent used for the leaves and fruits extraction were n-haxane, benzene, acetonic, eth...
Chemical constituents and pharmacological activities of Gossypium herbaceum and Gossypium hirsutum - A review
The phytochemical investigations of Gossypium herbaceum showed that it contained carbohydrates, flavonoids, tannins, steroids, terpenoids, saponins, resins, phenols and proteins, while, Gossypium hirsutum contained alkal...
Studies on the Effect of Diet and Drug Therapies on Serum Ferritin, a Predictor of Type 2 Diabetes Mellitus
Background and Objective: Epidemiological studies have established a link between serum ferritin concentration and type 2 diabetes. This work was carried out to ascertain the effect of therapeutic regimen on th...
The Effect Of Roselle Extract (Hibiscus sabdariffa Linn.) On Blood Glucose Level And Total Antioxidant Level On Diabetic Rat Induced By Streptozotocin
: Roselle (Hibiscus sabdariffa Linn.) contains phenolic compounds, especially anthocyanins which act as antioxidants. The purposes of this study were to examine the antioxidant capacity and capability of purple...