Development and Validation of a Stability Indicating RP-HPLCMethod for Determination of Ondansetron in Orally DisintegratingFilms
Journal Title: International Journal of Research in Pharmacy and Science - Year 2013, Vol 3, Issue 1
Abstract
A simple, precise, rapid and accurate stability indicating reversed-phase high performance liquidchromatography (RP-HPLC) method is developed for the estimation of ondansetron in orallydisintegrating films. The separation was achieved by using a Waters 2695 HPLC System consistingof analytical column Unisphere-C8 (5μm; 150x4.6mm) and wavelength detector- Waters 2489 UVis used for analysis. The mobile phase consisting of A- phosphate buffer (pH 5.4): acetonitrile in theratio of 72:28 (v/v) is used. The flow rate is 1.0 mlm-1 and the effluents are monitored at 247 nm.The retention time is about 5.0 m. The detector response is linear in the concentration range of 40.0-120.0 μgml-1. The respective linear regression equation being y = 30503 + 65831. The percentageassay of ondansetron is 100.6%. The method is validated as per ICH guideline by determining itsspecificity, accuracy, precision, linearity & range, ruggedness, robustness and system suitability.The results of the study show that the proposed method is simple, rapid, precise and accurate, whichis useful for the routine determination of ondansetron in its orally disintegrating films.
Authors and Affiliations
Pradeep Singh , Subhsh Dinda
Keywords
Related Articles
- EP ID EP151421
- DOI -
- Views 107
- Downloads 0
Development and Validation of A Stability Indicating RP-HPLC Method for Determination of Tapentadol in Tapentadol Hydrochloride Tablets
A simple, precise, rapid and accurate stability indicating reversed-phase high performance liquid chromatography (RP-HPLC) method is developed for the estimation of tapentadol in tapentadol hydrochloride tablets. The sep...
PHYSICOCHEMICAL PROPERTIES, FATTY ACIDS PROFILES AND ANTIOXIDANT PROPERTIES OF SEED OIL OF BREADFRUIT(TRECULIA AFRICANA).
Oil extracted from seed of Treculia Africana (breadfruit) was analyzed for physicochemical properties, free fatty acids profiles and antioxidant properties as part of an on-going screening process for plant constituents...
Synthesis & Antimicrobial Activity of 5-Arylidine Derivatives of n-(2-(4-Chlorophenyl)-4-Oxothiazolidin-3-yl) isonicotinamide
5-Arylidine derivatives of N-(2-(4-chlorophenyl)-4-oxothiazolidin-3-yl) isonicotinamide are synthesized using three steps: synthesis of N-(4-chlorobenzylidene isonicotinohydrazide, synthesis of 4- thiazolidinone ri...
The Opportunities and Challenges Involved in Registration of Similar Biotherapeutic Products in Emerging Countries
Although global biosimilars market looks attractive and continue to grow, the regulatory and operational hurdles remain in emerging regions. Many emerging nations are establishing biosimilars regulatory pathways, giving...
Development of HPTLC Qualitative Finger Printing Profile of AlmondOil in Marketed Herbal Cream
The almond oil is lightweight, golden-brown oil which is extracted from sweet almond nuts. Almondoil is derived form almond fruits; is a type of nut oil and used as natural remedy in many of the externaland i...