Development and Validation of a Stability Indicating RP-HPLCMethod for Determination of Ondansetron in Orally DisintegratingFilms
Journal Title: International Journal of Research in Pharmacy and Science - Year 2013, Vol 3, Issue 1
Abstract
A simple, precise, rapid and accurate stability indicating reversed-phase high performance liquidchromatography (RP-HPLC) method is developed for the estimation of ondansetron in orallydisintegrating films. The separation was achieved by using a Waters 2695 HPLC System consistingof analytical column Unisphere-C8 (5μm; 150x4.6mm) and wavelength detector- Waters 2489 UVis used for analysis. The mobile phase consisting of A- phosphate buffer (pH 5.4): acetonitrile in theratio of 72:28 (v/v) is used. The flow rate is 1.0 mlm-1 and the effluents are monitored at 247 nm.The retention time is about 5.0 m. The detector response is linear in the concentration range of 40.0-120.0 μgml-1. The respective linear regression equation being y = 30503 + 65831. The percentageassay of ondansetron is 100.6%. The method is validated as per ICH guideline by determining itsspecificity, accuracy, precision, linearity & range, ruggedness, robustness and system suitability.The results of the study show that the proposed method is simple, rapid, precise and accurate, whichis useful for the routine determination of ondansetron in its orally disintegrating films.
Authors and Affiliations
Pradeep Singh , Subhsh Dinda
Keywords
Related Articles
- EP ID EP151421
- DOI -
- Views 86
- Downloads 0
DPPH Scavenging Activity of Various Extracts of Sweet Potatoes Leaves with Varying Tubers Colors
The antioxidant activities of various extracts of sweet potatoes (Ipomoea batatas) leaves with five varying tubers colors were examined by using DPPH (2.2-diphenyl-1-picrylhidrazyl) method. Determination of total f...
Synthesis & Antimicrobial Activity of 5-Arylidine Derivatives of n-(2-(4-Chlorophenyl)-4-Oxothiazolidin-3-yl) isonicotinamide
5-Arylidine derivatives of N-(2-(4-chlorophenyl)-4-oxothiazolidin-3-yl) isonicotinamide are synthesized using three steps: synthesis of N-(4-chlorobenzylidene isonicotinohydrazide, synthesis of 4- thiazolidinone ri...
Pharmacognostic and Preliminary Physiochemical Investigations of Thevetia peruviana (Pers.) K. Schum Flowers
Flowers of Plant Thevetia peruviana (Pers.) K. Schum.were reported to possess good medicinal value in traditional system of medicine, the present investigation deals with Macroscopic, Microscopic and preliminary ph...
FORMULATION DEVELOPMENT AND ASSESSMENT OF CONTROLLED RELEASE BILAYERED OSMOTIC TABLET CARRYING SULFONYLUREA CLASS - ANTI DIABETIC AGENT & IMPERATIVE FACTORS IMPARTING SIGNIFICANT IMPACT ON DRUG RELASE
Osmosis is an aristocratic biophenomenon, which is exploited for development of delivery systems with every desirable property of an ideal controlled drug delivery system. Osmotic system utilizes the principles of osmoti...
Antimalarial Activity: A QSAR Modeling of NF54 Strain of Plasmodium falciparum by Physicochemical Descriptor Calculation
Multiple linear regression analysis and other statistical analysis were carried out on all the compounds of training set. Descriptors were selected for the model based on their correlation coefficient and those descripto...