Development and Validation of a Stability Indicating RP-HPLCMethod for Determination of Ondansetron in Orally DisintegratingFilms
Journal Title: International Journal of Research in Pharmacy and Science - Year 2013, Vol 3, Issue 1
Abstract
A simple, precise, rapid and accurate stability indicating reversed-phase high performance liquidchromatography (RP-HPLC) method is developed for the estimation of ondansetron in orallydisintegrating films. The separation was achieved by using a Waters 2695 HPLC System consistingof analytical column Unisphere-C8 (5μm; 150x4.6mm) and wavelength detector- Waters 2489 UVis used for analysis. The mobile phase consisting of A- phosphate buffer (pH 5.4): acetonitrile in theratio of 72:28 (v/v) is used. The flow rate is 1.0 mlm-1 and the effluents are monitored at 247 nm.The retention time is about 5.0 m. The detector response is linear in the concentration range of 40.0-120.0 μgml-1. The respective linear regression equation being y = 30503 + 65831. The percentageassay of ondansetron is 100.6%. The method is validated as per ICH guideline by determining itsspecificity, accuracy, precision, linearity & range, ruggedness, robustness and system suitability.The results of the study show that the proposed method is simple, rapid, precise and accurate, whichis useful for the routine determination of ondansetron in its orally disintegrating films.
Authors and Affiliations
Pradeep Singh , Subhsh Dinda
Keywords
Related Articles
- EP ID EP151421
- DOI -
- Views 121
- Downloads 0
Spectrophotometric Determination of Guaifenesin and Pseudoephedrine Hydrochloride in Tablet Dosage Form
Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of guaifenesin and pseudoephedrine hydrochloride in tablet dosage form have been developed....
RP – HPLC Simultaneous Estimation of Diclofenac Diethylamine and Lidocaine in Pharmaceutical Gel Formulation
A new specific, precise, accurate and robust RP-HPLC method has been developed for the simultaneous determination of Lidocaine and Diclofenac Diethylamine in a pharmaceutical gel formulation. The chromatographic sepa...
FORMULATION DEVELOPMENT AND ASSESSMENT OF CONTROLLED RELEASE BILAYERED OSMOTIC TABLET CARRYING SULFONYLUREA CLASS - ANTI DIABETIC AGENT & IMPERATIVE FACTORS IMPARTING SIGNIFICANT IMPACT ON DRUG RELASE
Osmosis is an aristocratic biophenomenon, which is exploited for development of delivery systems with every desirable property of an ideal controlled drug delivery system. Osmotic system utilizes the principles of osmoti...
Development and Validation of A Stability Indicating RP-HPLC Method for Determination of Tapentadol in Tapentadol Hydrochloride Tablets
A simple, precise, rapid and accurate stability indicating reversed-phase high performance liquid chromatography (RP-HPLC) method is developed for the estimation of tapentadol in tapentadol hydrochloride tablets. The sep...
An Update on Pharmacological Properties of Cumin
Cumin commonly known as Jeera commonly used in the house hold work as spice. Cumin(Cuminum cyminum) is a flowering plant in the family Apiaceae It is an ancient spice which history dates back to use in Egypt used by...