DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD OF LORNOXICAM FOR STABILITY INDICATING ASSAY
Journal Title: Asian J of Pharm Sci & Tech - Year 2014, Vol 4, Issue 1
Abstract
ABSTRACT Objective: The objective of the current study was to develop and validate a simple, accurate, precise and selective stability-indicating gradient reverse phase high performance liquid chromatographic method for estimation of Lornoxicam in pharmaceutical formulation in presence of degradation products. Material and methods: The chromatographic separation of Lornoxicam was achieved on Shimadzu LC-20AT series HPLC having C18-ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible detector at 371 nm. The optimized mobile phase was consisted of a Methanol : acetonitrile : Water (50:30:20) at a flow rate of 1.0 mL/min. The retention times were 7.212 min for Lornoxicam. Results and Discussion: The proposed method provided linear responses within the concentration ranges 5-25µg/mL for Lornoxicam. The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 0.0259µg/mL and 0.0206µg/mL for Lornoxicam. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision, robustness and ruggedness. Conclusions: The studies data revealed that developed method was convenient, fairly reliable, sensitive, less expensive and reproducible. Key words: RP-HPLC, Validation, Stability-indicating assay, Lornoxicam.
Authors and Affiliations
Pratiksha Shrivas, Nivrati Jain, Harshita Jain, Sunil Kumar Jain, Prateek Kumar Jain, Ashish Kumar Jain*
Keywords
Related Articles
- EP ID EP123312
- DOI -
- Views 136
- Downloads 0
FIVE-PARAMETER TYPE I GENERALIZED LOGISTIC DISTRIBUTION
ABSTRACT In this paper, we consider further generalization of the four-parameter type I generalized logistic distributions in Olapade (2004) to a five-parameter type I generalized logistic distribution. Some theo...
Pharmacological studies of anti diarrhoeal activity of Casuarina equisetifolia (L.) in experimental animals
ABSTRACT The purpose of the present study was to evaluate scientifically the anti-diarrhoeal effects of ethanolic (90%) extract of Casuarina equisetifolia Linn (EECE) was studied against castor oil-induced-diarrh...
REVIEW ON CONCEPTS OF POLYPILL
ABSTRACT Polypill (Combo pill) is a medication that is a drug product in pill form (i.e, tablet or capsule) that combines multiple active pharmaceutical ingredients. It is commonly manufactured as a fixed dose co...
A REVIEW ON SAFETY OF AYURVEDIC MEDICINE
ABSTRACT Ayurvedic medicine is a Hindu system of traditional medicine native to India and a form of alternative medicine. Ayurveda is an unscientific way of treatment. The earliest literature on Indian medical pr...
NEW RECORD OF COLONIAL ASCIDIAN POLYCITOR CALAMUS KOTT, 1992 OF THE FAMILY POLYCITORIDAE MICHAELSEN, 1904 FROM INDIAN WATERS
ABSTRACT Ascidians are common biofouling oraganisms found on almost all underwater marine structures and distributed from the lowest low tide level to the depth of the ocean. A biological resources study of ascid...