Development of primers for loop-mediated isothermal amplification of Philippine white spot syndrome virus isolates
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2012, Vol 5, Issue 4
Abstract
Because there are currently no effective chemicals or drugs to completely treat bacterial and viral diseases, effective means of prevention such as early detection methods are continuously being explored and fully exhausted. Over the past few years, we have seen the development of cutting edge technologies to specifically and efficiently detect White Spot Syndrome Virus (WSSV), one of the major viruses that devastated global shrimp aquaculture. The recent discovery of loop mediated isothermal amplification (LAMP) brought about opportunities for fast diagnosis of a lot of animal diseases including humans. Here, the development of alternative LAMP primers specifically for Philippine WSSV isolates was discussed. The sensitivity of the established detection protocol was found to be at 0.3954 pg of shrimp DNA template while exhibiting high specificity to the viral target. In addition, alternative visualization techniques and comparison with other detection protocols are also discussed
Authors and Affiliations
Benedict Maralit, Christopher Caipang, Mudjekeewis Santos, Adelaida Calpe, Mary Maningas
Keywords
Related Articles
- EP ID EP130505
- DOI -
- Views 81
- Downloads 0
Preliminary data on wintering habits of an intra-urban population of hybrid newts Triturus cristatus X Triturus dobrogicus (Caudata: Salamandridae)
The study focuses on an intra – urban population of Triturus cristatus x Triturus dobrogicus located in the Cismigiu Garden in Bucharest. The aim was to observe how microhabitat choice influences the success of overwinte...
The distribution of crayfish (Decapoda: Astacidae, Cambaridae) population in Cris and Mures rivers crossing the Romanian-Hungarian border
The crayfish (Decapoda) fauna of the Mures/Maros, Crisul Repede/Sebes-Körös, Crisul Negru/Fekete-Körös and Crisul Alb/Fehér-Körös rivers was surveyed in 16 sampling areas in 2012. The crayfish assemblages of the sampling...
Sorption kinetics of Pb, Cu and Zn ions by natural zeolites from Maramures County (northern Romania): potential application inwastewater reus
The sorption kinetics of Pb[sup]2+[/sup], Cu[sup]2+[/sup]and Zn[sup]2+[/sup]ions by natural zeolite tuff from Maramureş County (northern Romania) was studied using agitated batch laboratory apparatus at two temperature l...
Is intensification a viable way for pond culture in Central and Eastern Europe
Today’s Central and Eastern European aquaculture is based on low production intensity pond culture dominated by traditional extensive and semi-intensive carp-based polyculture technologies, use of complete feeds in pond...
Research concerning the feed digestibility and the digestive utilization coefficient in grass carp (Ctenopharingodon idella).
The experiment was organized to investigate the recovery of additional food (fodder and terrestrial vegetation) given to fish, Ctenopharyngodon idella, in particular through the study of nutrient digestibility. Studied f...