Development of primers for loop-mediated isothermal amplification of Philippine white spot syndrome virus isolates
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2012, Vol 5, Issue 4
Abstract
Because there are currently no effective chemicals or drugs to completely treat bacterial and viral diseases, effective means of prevention such as early detection methods are continuously being explored and fully exhausted. Over the past few years, we have seen the development of cutting edge technologies to specifically and efficiently detect White Spot Syndrome Virus (WSSV), one of the major viruses that devastated global shrimp aquaculture. The recent discovery of loop mediated isothermal amplification (LAMP) brought about opportunities for fast diagnosis of a lot of animal diseases including humans. Here, the development of alternative LAMP primers specifically for Philippine WSSV isolates was discussed. The sensitivity of the established detection protocol was found to be at 0.3954 pg of shrimp DNA template while exhibiting high specificity to the viral target. In addition, alternative visualization techniques and comparison with other detection protocols are also discussed
Authors and Affiliations
Benedict Maralit, Christopher Caipang, Mudjekeewis Santos, Adelaida Calpe, Mary Maningas
Keywords
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