Dimethyl 3, 3’, 4, 4’-tetrahydroxy-δ-truxinate isolated from the leavesof Andrographis lineata.Wall. ex. Nees suppress adipogenesis in 3T3-L1preadipocytes for type 2 diabetes
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 2
Abstract
The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of Andrographis lineata (EtALL) which suppress the differentiation of 3T3-L1 adipocytes. The ethanolic leaf extract was subjected to bioassay guided fractionation in 3T3-L1 cell lines. Five fractions were isolated from the EtALL extract by column chromatography. All the Fractions (I-V) along with EtALL were screened for adipogenesis activity (Oil-Red-O staining).The fraction which showed maximum adipogenesis activity was purified by thin layer chromatography. The bioactive Fraction IV was found to have maximum adipogenic (96.83%) activity and the activity was comparable to Rosiglitazone. The spectroscopic data analysis reveals that, the isolated bioactive compound was Dimethyl 3, 3’, 4, 4’-tetrahydroxy-δ-truxinate (DTδT), a combination of truxillic and truxinic acid derivative. DTδT showed insulin mimicking (131.2%), sensitizing (810.02%) and adipogenic activity (80.23%). Hence our present study concluded that, Dimethyl 3, 3’, 4, 4’-tetrahydroxy-δ-truxinate isolated from the ethanolic leaf extract of Andrographis lineata stimulates glucose uptake, potentiates insulin–stimulated glucose in 3T3-L1 adipocytes without increasing adiposity.
Authors and Affiliations
Vijayakumar Sudarshana Deepa , Krishnasamy Rajaram , Periyasamy Sureshkumar
Keywords
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