Effect of Freeze-dried cow urine of different Indian breeds in tertiary combinations with plant products on the reproduction and certain biochemical parameter in the ovotestis of Lymnaea acuminata
Journal Title: Applied Science Reports - Year 2013, Vol 1, Issue 2
Abstract
Present paper focus on the effect of sublethal treatment (20% and 60% of 24h LC50) of freeze-dried urine of different Indian breeds of cow such as Sahiwal, Geer and Tharparkar kept for 15 days in sunlight (8h/days) and laboratory condition of tertiary combination (1:1:5) with plant molluscicides such as Annona squamosa seed powder, Ferula asafoetida root latex, Azadirachta indica oil and Camellia sinensis leaves on the reproduction of snail Lymnaea acuminata. It was observed that these combinations caused a significant (P<0.05) reduction in the fecundity, hatchability and survival of young snails Lymnaea acuminata. Sublethal treatment with tertiary combination of freeze-dried cow urine increased the hatching period of snail with respect to control. Among all the treatments 60% of 24h LC50 caused no fecundity after 72h and no survival, after 48h exposure period. Sublethal treatment with freeze-dried cow urine of different breeds of cow and its tertiary combination caused significantly (P<0.05) changed in level of different biochemical parameters such as protein, amino acid, phospholipids, lipid per oxidation and acid/alkaline phosphatase activity in ovotestis of snail L. acuminata. Treatment with 60% of 24h LC50 of Tharparkar cow urine kept for 15 in sunlight + A. indica oil + C. sinensis caused maximum reduction in pr
Authors and Affiliations
Shiv Kumar D. K Singh , Vinay Kumar Singh
Keywords
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