Ex vitro multiple shoot regeneration potential of hypocotyls of four Rhizophoraceae mangroves
Journal Title: Scientia Agriculturae - Year 2016, Vol 14, Issue 1
Abstract
Day by day true mangrove species belonging to the family Rhizophoraceae occurring mangrove forests of Odisha coast, India are struggling hard for survival due to habitat loss and fragmentation. To arrest such problems, application of various scientific methods and techniques are needed to augment vegetative propagation systems with all Rhizophoraceae mangroves species. In this context, mass multiplication through shoot regeneration by decapitation from a single hypocotyl is a targeted tool. The present study revealed that the hypocotyls of four Rhizophoraceae mangroves viz. Kandelia candel, Bruguiera gymnorrhiza, Bruguiera parviflora and Rhizophora apiculata showed good potential for ex vitro shoot regeneration through decapitation and bud primordia formation from a histological point of view. Among four studied species, the best multiple shooting ability was recorded in the hypocotyls of K. candel at 40-Day of decapitation. The hypocotyl of K. candel gave rise to multiple shoots with 5.5±2.0 nos. per hypocotyl regenerated at 40-day followed by B. gymnorrhiza (4.0±1.5), B. parviflora (3.4±1.17) and R. apiculata (3.0±1.2). From histological analysis, at day-20 shoot primordia were found initiated from meristem cells in the region of the severed vascular bundles near the exposed surface of the hypocotyls whereas induced meristematic cells gave rise to vascular connected multiple shoots at 40-day of decapitation in case of B. parviflora. Similarly, shoot or bud primordia were developed with tunica layer at 20-day and fully developed shoot initials possessing vascular connection was recorded at 40-day in both B. gymnorrhiza and R. apiculata. Shoot or bud primordial with dense cytoplasmic cells was observed at day-20 and emerging shoot initials having vascular connection were noticed at day-40 in K. candel. The fact that additional bud primordia were present within the hypocotyls of Rhizophoraceae family suggests that further implementation of the hypocotyls is helpful to maximize the potentiality of shoot regeneration and conservation of the mangrove forest.
Authors and Affiliations
Pramodini Rout, Pradipta Kumar Mohapatra, Uday Chand Basak
Keywords
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- EP ID EP117807
- DOI 10.15192/PSCP.SA.2016.14.1.210215
- Views 104
- Downloads 0
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