High-level expression of fusion peptide Dybowskin-2CDYa and hEGF in E. coli and its dual function
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 3
Abstract
Dybowskin-2CDYa (Dy2), with a broad antimicrobial spectrum and low hemolytic feature, is a newly discovered type of antimicrobial peptides from Rana dybowskii. In order to get a dual function peptide which inhibits bacterial growth and promotes cell proliferation, we cloned the gene of Dy2and hEGF (human epidermal growth factor) into the prokaryotic expression vector pET-30a(+). With isopropyl-β-D-thiogalactoside (IPTG) induction, a 13.7KDa peptide with a 6×His tag was highly expressed in the form of inclusion in E. coli BL2l (DE3). SDS-PAGE and western-blot confirmed the expression of the fusion peptide hEGF-Dy2. Under the optimized condition of 1.0mmol/L IPTG induction and incubation time 4h at 37o, the yield of hEGF-Dy2reached 30mg/L following purification on nickel-nitrilotriacetic acid (Ni-NTA) metal affinity chromatography matrices. The purified fusion peptide showed significant antibacterial activities against Staphylococcus aureus, Escherichia coli O157, Pseudomonas aeruginosa and proliferating activities on NIH3T3. These results indicated that the fusion peptide might have a good prospect in therapy of trauma and burns.
Authors and Affiliations
LiLi Jin , Chao Jiang , XueMin Zhang , Zheng Wang , Xiao Hou , QiuYu Wang
Keywords
Related Articles
- EP ID EP99518
- DOI -
- Views 115
- Downloads 0
Plantago ovata: Clinical study of overuse
The objective of the study was to undertake evidence-base study to evaluate clinical manifestation of the over-estimated use of herbal drug Plantago ovata and to compare it with placebo for the efficacy and adverse...
Statistical study of the risk factors of myocardial infarction in the patients of district Muzaffarabad capital of Azad Jammu and Kashmir
Coronary artery disease (CAD) is a leading cause of mortality in the developing countries. The aim of the study was to check the association of Myocardial infarction (MI) with several factors such as smoking &...
Human Pappilomavirus (HPV) induced cancers and prevention by immunization
Incidences of different types of cancer are increasing in Pakistan, among which cancer of Cervix and Respiratory pappilomatosis are of great concern because of their association with human Pappilomavirus (HPV). Cervical...
Antihepatotoxic effect of golden berry (Physalis peruviana Linn.) in carbon tetrachloride (CCl4) intoxicated rats
Liver is the main site in the body for intense metabolism and excretion. A number of chemicals and drugs which are used routinely cause liver damage. The present study investigates the antihepatotoxic effect of Phy...
Evaluation of wound healing effects between Salvadora persica ointment and Solcoseryl jelly in animal model
In this research study very first time a herbal ointment contain 10% Salvadora persica extract was compared with Solcosseryl jelly 10% and blank Vaseline to evaluate wound healing effects using excision wound heali...