Immunomodulatory effects of turmeric, Curcuma longa (Magnoliophyta, Zingiberaceae) on Macrobrachium rosenbergii (Crustacea, Palaemonidae) against Vibrio alginolyticus (Proteobacteria, Vibrionaceae
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2012, Vol 5, Issue 1
Abstract
Macrobrachium rosenbergii is being favored for farming due to its large size. However, like other crustaceans, it is also prone to bacterial and viral infections. Turmeric, a derivative of the plant Curcuma longa, is a spice commonly used in Middle East and Asia as an herbal remedy. Immuno-modulatory effects of turmeric powder on hemocyte population and expression of antimicrobial peptides (AMPs) of M. rosenbergii challenged with Vibrio alginolyticus was investigated. Eighty (80) juveniles of M. rosenbergii were divided into three groups labeled as D0, D1 and D7 (Day 0, 1 and 7 respectively). D0 were fed with commercial feeds while D1 and D7 were fed with turmeric incorporated (enhanced) feeds for one and seven days respectively. The total hemocyte count (THC) of D0 remained constant and a significant increase was observed from D1 to D7 treatment. Prawns were challenged with V. alginolyticus and total RNA was isolated and synthesized into cDNAs from hepatopancreas. RT-PCR was performed with crustin and lysozyme as target genes and EF-1α as the reference gene. PCR products were run through 1% agarose gel electrophoresis. Semi-quantitative RT-PCR revealed an increasing expression of crustin and lysozyme PCR relative to duration of feeding, indicating a remarkable increase in the expression of AMPs (antimicrobial peptides). Challenged prawns fed with enhanced feeds also had an induced expression of AMPs. It is noteworthy to mention that this is the first report on AMPs expression in M. rosenbergii.
Authors and Affiliations
Jennifer Alambra, Rod Alenton, Pia Gulpeo, Christine Mecenas, Abigail Miranda, Rey Thomas, Maden Velando, Lawrence Vitug, Mary Maningas
Keywords
Related Articles
- EP ID EP161620
- DOI -
- Views 106
- Downloads 0
Ingestion induced health risk in surface waters near tailings ponds (North-Western Romania)
Anthropogenic activities generate most elements of environmental pollution, especially with heavy metals. The paper presents some investigations of several heavy metals (Pb, Cu, Cd, Zn) concentrations, performed on water...
Preliminary studies of quality assessment of aquatic environments from Cluj suburban areas, based on some invertebrates bioindicators and chemical indicators
Systematic categories of invertebrates bioindicators correlated with some chemical parameters, were an effective way to characterize the quality of lotic (Somes River) and lentic (Lake Gilau) aquatic environment from Clu...
Types of water pollution: point source and nonpoint source
Water is an important element to human life, this is why around water sources human settlements have been built which led to the development of these specific areas. One person needs a minimum of 5 L water per day, out o...
Errors in experimental design and statistical analysis of aquaculture diet evaluation studies induced by filtration systems.
While single factor diet studies appear to be of simple design, the space constraints in aquaculture caused by the need to filter the aqueous media often create constraints on the implementation of the experimental desig...
Effect of astaxanthin and cholesterol on growth, survival, and pigmentation of adult spiny lobster, Panulirus ornatus (Decapoda, Palinuridae).
Prior research on the spiny lobster Panulirus ornatus (Fabricius, 1798) determined that intermediate levels of cholesterol are important in diets, but astaxanthin may not be. Here we examined how the growth, survival and...