In situ and ex situ preservation of Prussian carp oocyte
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 4
Abstract
The decline of ova viability after ovulation is one of the limiting factors in controlled reproduction of several fish especially in warm water fish. The purpose of this study was evaluating the viability of Prussian carp oocytes retained within the ovarian cavity (in situ storage) and outside of theovarian cavity (ex situ storage) at 21C and 4 ْ ْC for 20, 30, 60 and 120 min. Experiments were performed with 20 female fish (10 females for each experimental group) Carassius gibelio (Bloch, 1782) induced to spawn with 0.5 mg kg[sup]1 [/sup]Ovaprim (sGnRH+Dompridon). Prussian carp oocytes could be successfully stored for 2 h in situ at 21C and 4 ْ C without significant decrease in eyed embryo and hatching rate (P>0.05). The percentage of eyed embryo in kept eggs outside the ovarian cavity at 21C and 4 ْ C for all durations was significantly different compared to control group (P<0.05). There was not significantly different in deformed larvae percent during in situ and ex situ storage at 21 ْC and 4 ْC as compared with control.
Authors and Affiliations
Mohammad Taati, Ali Shabani, Mohammad Imanpoor
Keywords
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