In vitro antimicrobial, antioxidant activity and phytochemical screening of Apium graveolens
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 5
Abstract
The present study evaluates the phytochemical screenings, antioxidant activity and antimicrobial assay of Apium graveolens L. The phytochemical screening showed the presence of flavonoids, tannins, saponins and steroids in Apium graveolens while terpenoids was absent. The total phenolic content was slightly higher in methanolic fraction (63.46±12.00mg GAE/g) followed by ethanol (36.60±12.28 mg GAE/g) and hexane fractions (34.86±6.96mg GAE/g). The flavonoid content was high in methanolic extract (56.95±7.14mg Quorcetin/g) and low level of the content was found in methylated spirit extract (29.2±3.15mg Quercitin/g). Antioxidant activity assayed by FRAP was higher in methanolic fraction (12.48±1.06 mmole of FeSO4 equivalent/litre of extract) compared with other extracts. Likewise, good antimicrobial activity was measured by crude ethanol fraction against S. aureus (MIC=0.12±0.03µg/ml) and S. typhi (MIC= 0.5±0.2µg/ml). Results also that ethanolic fraction was effective against A. flavus (MIC= 05±1.0µg/ml).
Authors and Affiliations
Zakir Ud Din , Anwar Ali Shad , Jehan Bakht , Inam Ullah , Saleem Jan
Keywords
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