Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties
Journal Title: Journal of BioScience and Biotechnology - Year 2014, Vol 3, Issue 1
Abstract
Leuconostoc mesenteroides NRRL B-1149 was evaluated for its probiotic properties. It displayed in vitro cell surface traits in terms of hydrophobicity, autoaggregation property and cell adhesive capacity on HeLa, murine macrophage and HT29 cell lines, which revealed its capability to adhere and colonize the intestine. The tolerance to various biological barriers such as lysozyme (100 μg/ml), gastric juice (pH 3.0) and bile salts (0.5%, w/v) with 92%, 61.6% and 219% growth, respectively, confirmed its ability to survive in extreme conditions of digestive tract. It showed bile salts hydrolase activity signifying its ability to deconjugate bile salts. The whole cells of Leuconostoc mesenteroides B-1149 showed β-galactosidase activity with 94 Miller units displaying its importance in lactose utilization. The effect of dextran from Leuconostoc mesenteroides B-1149 having α-(1,6) linear glycosidic linkages and α-(1,3) branching was evaluated against cervical cancer (HeLa) and colon cancer (HT29) cell lines. The dextran from L. mesenteroides B-1149 (100 μg/ml) showed 25% inhibition of HeLa cell lines and 1000 μg/ml dextran displayed 40% inhibition of HT29 cell lines. The effect of dextran on murine monocyte-macrophage line (J774A.1) showed increase in growth of macrophages confirming its biocompatible nature.
Authors and Affiliations
Rishikesh Shukla, Ilia Iliev, Arun Goyal
Keywords
Related Articles
- EP ID EP136507
- DOI -
- Views 88
- Downloads 0
The effect of olive cake types on lipase production by isolated Rhizopus sp. and process statistical optimization
The aim of this work was to study the production of extracellular lipase by solid-state fermentation with different olive cakes varieties including Mary, Shenghe and Yellow from isolated fungi using agro-industries waste...
Influence of composition on the biological activity of pectic polysaccharides from leek
Water-soluble and acid-soluble pectic polysaccharides with significant immunostimulating activity were isolated from alcohol-insoluble solids (AIS) of leek. Pectic polysaccharides characterized with high anhydrouronic ac...
Effect Of Agromorphological Diversity and Botanical Race on Biochemical Composition in Sweet Grains Sorghum [Sorghum bicolor (L.) Moench] of Burkina Faso
Sorghum bicolor (L.) Moench is an under-harvested crop in Burkina Faso. It is grown mainly for its sweet grains in the pasty stage. However, the precocity of the cycle and the sweet grains at pasty stage make it an inter...
Genomic changes in speciation of the family Chironomidae, Diptera
The mode of speciation adopted by a species or group of related species of animals (insects) is clearly determined to a certain extent by the architecture of their genetic system. Chromosome rearrangements, the loc...
Chemical composition and antibacterial activity of Juniperus thurifera L. essential oils
The qualitative and quantitative composition of the essential oils obtained from male and female leaves of Juniperus thurifera L., (growing in Algeria) has been investigated for the first time. The essential oils w...