Multicomponent spectrometric assay of cyanocobalamin and its photoproduct hydroxocobalamin in the presence of ascorbic acid in photolyzed solutions
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 2
Abstract
Abstract: The simultaneous determination of cyanocobalamin (CC), hydroxocobalamin (HC) and ascorbic acid (AA) in aqueous solution has been achieved by a multicomponent spectrometric method. CC undergoes photolysis in acidic and alkaline media to form HC and the reaction is enhanced in the presence of AA. The method has been used to evaluate the kinetics of photodegradation reactions of the vitamin. CC, HC and AA present in the photolyzed solutions have been determined by absorbance measurement at 550, 525 and 265 nm at pH 4.0. These wavelengths correspond to the absorption maxima of the three substances and thus provide high specificity and sensitivity to the method. The method has been validated with respect to various parameters relating to the analytical performance characteristics. The recovery of the method for the three compounds ranges from 97.1-103.0% with a RSD value of ±3%. The accuracy of the method is shown by the linearity of the kinetic plots in the concentration range studied. The method is simple, rapid and convenient for the proposed work.
Authors and Affiliations
Iqbal Ahmad, Kiran Qadeer, Ambreen Hafeez, Rahila Bano Bano, Faiyaz HM Vaid
Keywords
Related Articles
- EP ID EP93949
- DOI -
- Views 106
- Downloads 0
Clinical application of combined anesthesia in cesarean section
This study chose 60 cases of cesarean section for patients with epidural anesthesia (EA) combined spinal epidural anesthesia (CSEA) surgery for clinical application research. Compared with EA, the CSEA could work w...
Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran
Essential oil from flowers, stems, and roots of Eremostachys laevigata Bunge. gathered in Iran was analyzed using gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS), and 23, 21, and 9 compounds were...
In vitro antioxidant activity of methanolic extracts of various parts of Leptadenia pyrotechnica (Forssk.) Decne.
Leptadenia pyrotechnica is a desert plant and its unripe fruits are cooked as a vegetable. Besides, this plant is also used for treating various ailments by the dwellers, yet very little is known about its free rad...
Anxiolytic and hyperlocomotive effects of aqueous extract of Nigella sativa L. seeds in rats
Use of the herbal drugs increasing all over the world due to its minimum side effect. Nigella sativa black seeds used in folk medicine for the promotion of good health and for the treatment of many diseases .The pr...
The acetylcholinesterase (AChE) inhibition analysis of medaka (Oryzias latipes) in the exposure of three insecticides
The continuous effects on Acetylcholinesterase (AChE) activity of medaka (Oryzias latipes) caused by dichlorvos, methomyl and deltamethrin in vivo were investigated, and the trends of AChE activity inhibition due t...