Optimization of enzyme complexes for efficient hydrolysis of corn stover to produce glucose
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 3
Abstract
Hydrolysis of cellulose to glucose is the critical step for transferring the lignocellulose to the industrial chemicals. For improving the conversion rate of cellulose of corn stover to glucose, the cocktail of celllulase with other auxiliary enzymes and chemicals was studied in this work. Single factor tests and Response Surface Methodology (RSM) were applied to optimize the enzyme mixture, targeting maximum glucose release from corn stover. The increasing rate of glucan-to-glucose conversion got the higher levels while the cellulase was added 1.7μl tween-80/g cellulose, 300μg β-glucosidase/g cellulose, 400μg pectinase/g cellulose and 0.75mg/ml sodium thiosulphate separately in single factor tests. To improve the glucan conversion, the β-glucosidase, pectinase and sodium thiosulphate were selected for next step optimization with RSM. It is showed that the maximum increasing yield was 45.8% at 377μg/g cellulose Novozyme 188, 171μg/g cellulose pectinase and 1mg/ml sodium thiosulphate.
Authors and Affiliations
Xiaoxiao Yu , , Yan Liu , Jiatong Meng , Qiyue Cheng , Zaixiao Zhang , Yuxiao Cui , Jiajing Liu , Lirong Teng , Jiahui Lu , Qingfan Meng , Xiaodong Ren
Keywords
Related Articles
- EP ID EP105936
- DOI -
- Views 116
- Downloads 0
Clinical research for curing ankylosing spondylitis through combining etanercept, thalidomide and sulfasalazine
This article is to explore the curative effect of treating ankylosing spondylitis (AS) through combining etanercept, thalidomide and sulfasalazine. Sixty-two patients with AS were divided into 3 groups: experimenta...
Preparation, biodistribution and scintigraphic evaluation of 99mTc-lincomycin
A complex of lincomycin was synthesized with technetium-99m. The synthesis was carried out by using SnCl2.2H2O as reducing agent and ascorbic acid as stabilizer. The effect of various parameters such as amount of l...
Pharmacokinetics of fexofenadine in healthy Taiwanese volunteers
The aim of presented study was to assess pharmacokinetic properties of fexofenadine in Taiwanese volunteers. Thirty-three healthy male subjects received 180mg fexofenadine. Blood samples were drawn at appropriate t...
Anti-hyperglycemic and antinociceptive activity of methanol leaf and stem extract of Nypa fruticans wurmb
Nypa fruticans Wurmb. (Arecaceae) is a mangrove palm well-known for its traditional uses by the local practitioners against different ailments in southern regions of Bangladesh. However, the plant is yet to be scientific...
Evaluation of antioxidant potential and HPLC based identification of phenolics in Polygonum amplexicaule extract and its fractions
There is a growing interest for the plant-based medicines in pharmaceutical industry. Plant derived Antioxidants have gained huge importance regarding their medicinal value. The present study was designed to establ...