Optimum Conditions for Alginaseby Bacilllus Circulans R Isolate
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2015, Vol 5, Issue 1
Abstract
: Fourteen isolated bacteria same species of Bacillus which were taken from hospital Ramadi and Fallujah sample were collected during the period from October to December 2013 and cultured on blood agar to test the ability to lysis to measure the inhibition zone . six isolate were selected for production of alginase enzyme when cultured in alginate yeast extract broth then only one which have high efficient in the production of alginase enzyme was selected after diagnoseddependingonphenotypic,microscopic and biochemical tests which show that the isolate was Bacillus circulans R. The optimal conditions for alginase enzyme production were determined; the optimum incubation period was 24 hrs which gave enzymatic activity (106.1U/ml). 6x107cell/g dry weight was the optimum inoculums percentage which gave activity (111 Unit/ml), the optimum carbon source which gave activity (212.86 U/ml), when used sodium alginate while peptone was the optimum nitrogen sources with enzymatic activity (275 U/ml ). pH 7.5 gave maximum activity (211.63 U/ml) , the shaking incubator was best in the production of the enzyme which gave enzymatic activity (210 U/ml).
Authors and Affiliations
EssamFadel Al-Jumailyand RafalIsmaeel Ali
Keywords
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