Potent AChE enzyme inhibition activity of Zizyphus oxyphylla: A new source of antioxidant compounds
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 6
Abstract
The purpose of this study was to assess the antioxidant potential and enzyme inhibition of various fractions of Zizyphus oxyphylla. The plant metabolites were extracted in methanol and partitioned with n-hexane, chloroform, ethyl acetate and n-butanol successively. Phytochemical screening showed presence of alkaloids, terpenoids and flavonoids in ethyl acetate and n-butanol fractions. The antioxidant potential and acetylcholine esterase assay of all these fractions and remaining aqueous fraction was evaluated by using reported methods. The results revealed that chloroform soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 95.01±0.37% inhibition of DPPH radical at a concentration of 120µg/mL. The IC50 of this fraction was 13.20±0.27µg/mL, relative to butylated hydroxytoluene (BHT, a reference standard), having IC50 of 12.10±0.29µg/mL. It also showed highest total antioxidant activity i.e. 1.723±0.34 as well as highest FRAP value (339.5±0.57TEµM/mL) and highest total phenolic contents (142.65±1.20 GAE mg/g) as compared to the other studied fractions. The fractions were also studied for Acetylcholine esterase enzyme (AChE) enzyme inhibition activity and n-butanol soluble fraction exhibited maximum inhibition (95.5±0.13mg/mL with IC50 =9.58±0.08mg/mL relative to galanthamine (13.26±0.73mg/mL), while n- hexane soluble fraction (165.15±0.94mg/mL) showed non-significant. We are still working to isolate pure compounds for active fractions targeting potent inhibition responsible for some activities.
Authors and Affiliations
Farhana Mazhar , Raisa Khanum , Muhammad Ajaib , Muhammad Jahangir
Keywords
Related Articles
- EP ID EP106612
- DOI -
- Views 69
- Downloads 0
Activity directed investigation on anti-inflammatory fractions and compounds from flowers of Trollius chinensis
Abstract: The flower of Trollius chinensis Bunge is used as an anti-inflammatory drug to treat upper respiratory tract infection, pharyngitis, tonsillitis, and bronchitis. In order to identify the active componen...
The Fructose mystery: How bad or good is it?
This review article endeavoured to ascertain the facts about fructose, a mono-saccharide sugar being increasingly used in daily diet. Human and animal studies suggest that fructose ingestion produces deleterious effects...
Effect of repeated oral therapeutic doses of methylphenidate on food intake and growth rate in rats
Central nervous system stimulants are known to produce anorexia. Previous data suggest that methylphenidate can have variable effects on caloric intake and growth rate. A dose-response study was performed to monito...
Clinical research for curing ankylosing spondylitis through combining etanercept, thalidomide and sulfasalazine
This article is to explore the curative effect of treating ankylosing spondylitis (AS) through combining etanercept, thalidomide and sulfasalazine. Sixty-two patients with AS were divided into 3 groups: experimenta...
Pyrus pashia: A persuasive source of natural antioxidants
Pyrus pashia Buch. & Ham. was subjected to extraction with methanol. Methanolic extracts of fruit, bark and leaf were partitioned separately with four organic solvents in order of increasing polarity, asn-hexan...