Preliminary phytochemical screening, antimicrobial and antioxidant activities of Euphorbia milli
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 4
Abstract
Euphorbia milii is a Pakistani herb used for various infectious diseases. In this study we have carried out phytochemical, antibacterial and antioxidant investigation of different extracts/fractions. Phytochemical studies showed the presence of cardiac glycosides, steroids/phytosterols, anthocyanin, proteins, terpenoids, flavonoids and tannins. Susceptibility testing by well diffusion assay of its chloroform and methanol fractions revealed good antimicrobial activity against Klebsiella pneumonia and Staph epidermis. Ethyl acetate fraction of roots also exhibited considerable antimicrobial activity against most of tested pathogens. Various fractions (Hexane, chloroform, methanol and water) of E. milii were screen for their antioxidant potential using DPPH radical scavenging assay at different concentrations among these, chloroform fraction exhibited good scavenging activity. The IR spectroscopy of the various extracts/fractions indicated the presence of OH, saturated CH stretching, C=C, C=O, NO2, C-N, Ar-O, C-O- and R-O-Stretching respectively. The findings provide helpful evidence for the use of E. milii in traditional medicines.
Authors and Affiliations
Abdur Rauf , Ajmal Khan , Nizam Uddin , Muhammad Akram , Mohammad Arfan , Ghias Uddin , Muhammad Qaisar
Development paths of China’s agricultural Pharmaceutical industry under Eco-agriculture background
Using pesticides has double effects. On one hand, it contributes to pests control and regulates the growth of crops; On the other hand, it does harm to the environment. To develop ecological agriculture should not...
Clinical curative effect of Percutaneous vertebroplasty combined with 125I-seed implantation in treating spinal metastatic tumor
This paper selected and studied 15 in-hospital patients to analyze and discuss the clinical curative effect of percutaneous vertebroplasty (PVP) combined with 125I-seed implantation in treating spinal metastatic tu...
Plantago ovata: Clinical study of overuse
The objective of the study was to undertake evidence-base study to evaluate clinical manifestation of the over-estimated use of herbal drug Plantago ovata and to compare it with placebo for the efficacy and adverse...
Development and validation of HPLC analytical method for Nepafenac in ophthalmic dosage form (suspension)
The aim of the present study was to develop and validate an analytical method for the estimation of nepafenac as a raw material as well as in dosage form (suspension) by using reverse phase high performance liquid...
Synthesis, characterization and biological evaluation of tryptamine based benzamide derivatives Kiran
Benzamides and tryptamine are biologically significant compounds, therefore, various benzamide analogous of tryptamine have been efficiently synthesized using tryptamine and different benzoyl chlorides, in order t...