Quantification of glycyrrhizin in anti-stress herbal formulations by validate HPTLC method: A rational paradigm towards quality control of herbals
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 1
Abstract
In the present study an analytical method of high-performance thin-layer chromatography (HPTLC) has been developed for quantification of glycyrrhizin for marketed antistress liquorice root capsules (LRC) and herbal tea (HT). Chromatography was performed by using mobile phase ethyl acetate (EA): glacial acetic acid (GAA): Methanol (MeOH): water (H2O) in proportion of 6:2:2:1, v/v/v/v. The developed plate was scanned and quantified densitometrically at absorption maxima 254nm. The method was validated for various analytical parameters viz. precision, accuracy, recovery, robustness, specificity, detection and quantification limits. The developed system was found to give compact spot for glycyrrhizin (Rf = 0.33±0.001). The linearity relationship was described by the equation Y=6.841X+ 70.428. The limit of detection (34 ng band-1), limit of quantification (101ng band-1), recovery (99.4-99.8%), and precision (≤1.84% and ≤1.62%; intraday and interday, respectively) were found satisfactory for glycyrrhizin. Linearity range for glycyrrhizin was 100-600ng (r2=0.998). The amount of glycyrrhizin was estimated by comparing the peak area of standard and the same was present in crude extract. The content of glycyrrhizin was estimated as 11.4% and 4.7% w/w in sample LRC and HT, respectively. The proposed method will be useful to quantify the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug.
Authors and Affiliations
Nasir Ali Siddiqui , Perwez Alam , Mohammad Khalid Parvez , Omer Ahmed Basudan , Mohammed Salem Al-Dosari , Adnan Jathlan Al-Rehaily , Mohammed Fahad Al-Ajmi , Faiyaz Shakeel
Keywords
Related Articles
- EP ID EP111073
- DOI -
- Views 99
- Downloads 0
Evaluation of nutritional and sub-acute toxicological study of plant based supplement of Achyranthes aspera
The present study was conducted for the nutritional, microbiological and toxicological evaluation of test compound having main ingredient Achyranthes aspera. Nutritional value assessment, microbiological analysis a...
Haplophytin B from Maclurodendron porteri
An alkaloid from Maclurodendron porteri has been isolated and characterized. Extraction process was conducted by acid-base extraction method followed by column chromatography. The structure was established by nuclear mag...
Antidepressant activity of ethanolic extract of Hibiscus rosa sinenesis Linn.
The present study was undertaken to assess the antidepressant effect of crude ethanolic extract of floral part of Hibiscusrosa sinensis (HRS) at doses 100mg/kg, 250mg/kg and 500mg/kg using three parameters which ar...
Enhancement of sodium taurocholate to the absorption of cefquinome
The objective of the research was to detect the enhancement effect of sodium taurocholate on the absorption of cefquinome both in Caco-2 cells and rats. The absorption efficiency of cefquinome was determined by hig...
Antimicrobial potentials of Mentha longifolia by disc diffusion method
This study was conducted for the assessment of the antimicrobial activities of different solvents extracted samples from the aerial parts of Mentha longifolia against ten microbial species through the disc diffusio...