Reducing the Cytotoxicity of Poly-(L)-Lysine for Developing an Effective Gene Transfection System
Journal Title: International Journal of Pharmaceutical and Biological Sciences Fundamentals (www.ijpbsf.com) - Year 2012, Vol 2, Issue 1
Abstract
Gene therapy as a therapeutic treatment to genetic or acquired disease is attracting much interest in the research community, leading to noteworthy development over the past two decades. Although, this field is still dominated by viral vectors, non viral vectors have recently received an ever increasing attention in order to overcome the safety problems of their counterpart. Viral vectors, though highly efficient transfection agents poses risk of hypersensitive response as well as potential risk of insertion of the foreign gene in the genome of the organism. On the other hand, non viral vectors including lipids and cationic polymers are immunologically inert, can carry greater variety of cargo, are easier to produce and modify chemically. Thus, cationic polymers come out to be ideal candidate for non viral vectors in gene therapy. Poly (L-lysine) polymers, liner polypeptides with lysine as the repeat unit, were one of the first cationic polymers employed for gene transfer. Poly (Llysine)- based polymers, pioneered in 1987, was used for gene delivery by employing a targeting ligand and folate to facilitate receptor-mediated uptake. Increasing the molecular weight of PLL rendered the PLL-DNA complex toxic to the cells.The present study focuses on the bringing improvements on PLL system for a non viral vector for gene delivery that is low on toxicity and immunologically inert.
Authors and Affiliations
Gaurav Rana , Kashyap Kumar Dubey
Keywords
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