RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND TELMISARTAN IN TABLET DOSAGE FORM
Journal Title: Int J of Pharm Res & Analy - Year 2013, Vol 3, Issue 1
Abstract
A simple, sensitive and rapid reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine besylate and Telmisartan. A Phenominex-luna C18 column (250x4.6 mm i.d 5μ) was used with a mobile phase containing a mixture of acetonitrile and phosphate buffer in the ratio of 56:44%v/v.pH was adjusted with orthophosphoric acid to 4.The flow rate was 1ml/min and the eluents were monitored at the detector wavelength of 236nm.The retention times of Amlodipine besylate and Telmisartan were found to be 4.32 and 5.32 minutes respectively.The validation of the proposed method was carried out for its specificity, accuracy, pecision, linearity, limit of detection and limit of quantification for both Amlodipine and Telmisartan.
Authors and Affiliations
Rajeswari A , Vasanth PM , Ramesh T , Ramesh Malothu
Keywords
Related Articles
- EP ID EP152690
- DOI -
- Views 135
- Downloads 0
DEVELOPMENT OF UV SPECTROSCOPIC METHOD FOR THE DETERMINATION OF GUAIFENESIN IN BULK AND FORMULATION
The present research work discusses the development of UV Spectroscopic method for the estimation of Guaifenesin. Simple, specific, accurate and cost effective spectroscopic method has been developed for the estimation o...
AN OVERVIEW OF ELECTRON PROBE MICROANALYSIS
Electron Probe Microanalysis (EPMA) is an analytical technique that is used to establish the composition of small areas on specimens. It’s one of the several particle-beam techniques These analytical technique has...
SEASONAL WATER QUALITY ASSESSMENT OF SURFACE WATER IN DURG REGION
An investigation was undertaken to determine the Quality of surface water of Shivnath River & ponds in durg and rajnandgaon region”. The study assessed the evolution of water quality in Surface of Durg and Rajn...
HERBAL DRUGS AS ANTI-TUBERCULAR AGENTS – A REVIEW
ABSTRACT Tuberculosis (TB) is a disease that has affected mankind from very ancient times.The Anti-Tuberculosis (Anti-TB) drugs are less effective because of emergence of Multi-Drug Resistance (MDR) and extensive...
METHOD DEVELOPMENT AND VALIDATION OF OLMESARTAN, AMLODIPINE AND HYDROCHLOROTHIAZIDE IN COMBINED TABLET DOSAGE FORM
A simple, accurate, precise, economical and reproducible method was developed for simultaneous estimation of olmesartan, amlodipine and hydrochlorothiazide in bulk and its dosage form. The excipients in the c...