RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF GABAPENTIN AND METHYLCOBALAMIN IN TABLET DOSAGE FORMS
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 7
Abstract
ABSTRACT A simple, accurate, economical and reproducible HPLC method for simultaneous estimation of two component drug mixture of Gabapentin and Methylcobalamin (MCB) in combined tablet form have been developed. The detection was performed at 271 nm. The retention time of Gabapentin and Methylcobalamin was found to be 2.5 min and 3.08 min respectively. Linearity was observed in concentration range of 600-1800mcg/ml of Gabapentin and 1-3mcg/ml of Methylcobalamin. The reverse phase chromatographic method used C18 column and 0.1% Orthophosporic acid:acetonitrile in ratio of 55:45 as mobile phase. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, and robustness. Keywords: Gabapentin, Methylcobalamin, HPLC, Validation, Reverse Phase.
Authors and Affiliations
Shashe Kumar P*, Ramamohan Reddy T, Umamaheshwara Rao V
THREE SIMPLE VALIDATED UV SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF TIMOLOL MALEATE AND BRIMONIDINE TARTRATE AND THEIR COMPARISON USING ANOVA
ABSTRACT Timolol Maleate and Brimonidine Tartrate combination is used for the topical treatment of intra-occular pressure in patients of glaucoma. Three rapid, simple, accurate and precise UV spectrophotometric met...
DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF TENOFOVIR DISOPROXIL FUMARATE, LAMIVUDINE AND EFAVIRENZ IN BULK AND TABLET DOSAGE FORM
ABSTRACT A simple, sensitive, rapid, economic and accurate first order derivative spectrophotometric method has been developed for estimation of Tenofovir disoproxil fumarate (TDF), Lamivudine (LAM) and Efavirenz...
RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND TELMISARTAN IN TABLET DOSAGE FORM
ABSTRACT A simple, sensitive and rapid reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine besylate and Telmisartan. A Phenominex-luna C18 column (250x4.6 mm i.d 5µ) was used wit...
GREEN ANALYTICAL CHEMISTRY
ABSTRACT Analytical methods are developing rapidly, and it is considered to be a small-scale activity, but this is not always true in the case of controlling and monitoring laboratories whose number of runs perfo...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ETHINYL ESTRADIOL AND LEVONORGESTREL
ABSTRACT A simple, accurate, precise, sensitive, specific and reliable stability indicating RP-HPLC method was developed for simultaneous estimation of Ethinyl estradiol (EE) and Levonorgestrel (LEV) in Pharmaceuti...