THREE SIMPLE VALIDATED UV SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF TIMOLOL MALEATE AND BRIMONIDINE TARTRATE AND THEIR COMPARISON USING ANOVA
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 3
Abstract
ABSTRACT Timolol Maleate and Brimonidine Tartrate combination is used for the topical treatment of intra-occular pressure in patients of glaucoma. Three rapid, simple, accurate and precise UV spectrophotometric methods have been developed namely simultaneous equation method (Method 1), First Derivative Method (Method 2) and Ratio First Derivative Method (Method 3) for the simultaneous estimation of Timolol Maleate (TM) and Brimonidine Tartrate (BRT). In Method 1, Timolol Maleate and Brimonidine Tartrate exhibit good linearity over the concentration range of 2-50 μg/ml and 2-14 μg/ml respectively at 294 nm and 255 nm wavelengths. Method 2 is based on First Derivative Method, the ZCP of TM selected for the estimation of BRT is 294.3 nm and the ZCP of BRT selected for the estimation of TM is 302.3 nm. Linearity range is 2-50 μg/ml and 2-14 μg/ml for TM and BRT respectively. Method 3 is based on Ratio First Derivative Spectrophotometry. Linearity range is 2-50 μg/ml and 2-14 μg/ml at 290 nm and 244 nm for TM and BRT respectively. The proposed methods were validated according to the ICH guidelines for the evaluation of accuracy, precision and sensitivity. The three methods were compared using one-way ANOVA and the fcal value was found to be less than ftab value indicating that there is no significant difference in the assay results by the three methods. Thus, the proposed methods are rapid, accurate, precise and economically viable for the simultaneous estimation of TM and BRT respectively in pure form as well as in ophthalmic formulation. Keywords: Timolol Maleate, Brimonidine Tartrate, Simultaneous Equation method, First Derivative Method, Ratio First Derivative Spectrophotometry, Validation, ANOVA.
Authors and Affiliations
Heta H. Desai* and Anandkumari D. Captain
Keywords
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