Screening for potential α-Glucosidase inhibitors in Coptis chinensis franch extract using ultrafiltration LC-ESI-MSn
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 6
Abstract
Naturally existing α-glucosidase inhibitors from traditional herbal medicines have attracted considerable interest to treat type 2 diabetes mellitus (T2DM). Hundreds of herbs have been reported to have the potential to inhibit α-glucosidase. However, most common methods to examine the inhibitors of α-glucosidase are usually time-consuming. In the current study, the screening of α-glucosidase ligands from Coptis chinensis Franch extract was undertaken by ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry (ultrafiltration LC-ESI-MSn). Resultantly, the enzyme inhibition studies showed that Coptis chinensis Franch extract carries the strongest α-glucosidase inhibitory activity among the five kinds of Chinese herbal extracts. Subsequently, five compounds that could bind to α-glucosidase in the Coptis chinensis Franch extract were found using ultrafiltration liquid chromatography, and their structures were identified by ESI-MSn to be coptisine, epiberberine, jatrorrhizine, berberine, palmatine. Cumulatively, these results were anticipated to be encouraging for applying the Coptis chinensis Franch extracts as efficient anti-diabetic drug candidates.
Authors and Affiliations
Hui Zhou , Tao Jiang , Zhengyang Wang , Shen Ren , Wei Wu , Lianhai Jiang , Zhiqiang Liu , Lirong Teng
Keywords
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