Taguchi’s experimental design for optimizing the production of novel thermostable polypeptide antibiotic from Geobacillus pallidus SAT4
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 1
Abstract
Polypeptide antimicrobials used against topical infections are reported to obtain from mesophilic bacterial species. A thermophilic Geobacillus pallidus SAT4 was isolated from hot climate of Sindh Dessert, Pakistan and found it active against Micrococcus luteus ATCC 10240, Staphylococcus aureus ATCC 6538, Bacillus subtilis NCTC 10400 and Pseudomonas aeruginosa ATCC 49189. The current experiment was designed to optimize the production of novel thermostable polypeptide by applying the Taguchi statistical approach at various conditions including the time of incubation, temperature, pH, aeration rate, nitrogen, and carbon concentrations. There were two most important factors that affect the production of antibiotic including time of incubation and nitrogen concentration and two interactions including the time of incubation/pH and time of incubation/nitrogen concentration. Activity was evaluated by well diffusion assay. The antimicrobial produced was stable and active even at 55°C. Ammonium sulphate (AS) was used for antibiotic recovery and it was desalted by dialysis techniques. The resulted protein was evaluated through SDS-PAGE. It was concluded that novel thermostable protein produced by Geobacillus pallidus SAT4 is stable at higher temperature and its production level can be improved statistically at optimum values of pH, time of incubation and nitrogen concentration the most important factors for antibiotic production
Authors and Affiliations
Syed Aun Muhammad , Safia Ahmed , Tariq Ismail , Abdul Hameed
Keywords
Related Articles
- EP ID EP120827
- DOI -
- Views 53
- Downloads 0
Tissue distribution study of salvianolic acid B long-circulating liposomes in mice by UPLC-MS/MS determination
In targeting delivery system research on salvianolic acid B, it’s vital but hard to evaluate the tissue distribution for its low concentrations in tissues. So the simple, rapid, selective and sensitive UPLC-MS/MS m...
Unpredictable chronic mild stress induced behavioral deficits: A comparative study in male and female rats
Stress is an important precipitant factor for depression. Changes in various body systems that occur in depression are similar to those observed in response to stress. Chronic stress may alter behavioral, neurochem...
Anti-inflammatory and gastroprotective activities of the aqueous extract of Micromeria fruticosa (L.) Druce ssp Serpyllifolia in mice
Micromeria fruticosa is used widely in many Mediterranean regions for various inflammatory conditions. The aim of this work was to assess the anti-inflammatory and gastroprotective activities of the aqueous extract...
DFT for exploring the antioxidant potential of homogentisic and orsellinic acids
In recent years, much attention has been focused on the antioxidant potential of different phenolic acids. But still no theoretical investigation is reported on the antioxidant potential of Homogentisic and Orselli...
Synthesis, spectral characterization and enzyme inhibition studies of different chlorinated sulfonamides
Sulfonamides are adherent to a biologically dynamic category of compounds and are under consideration of many organic synthetic researches to synthesize pharmacologically important compounds. In this demonstrated r...