Simple and effective HPLC method development and its validation for clindipine in human drug free plasma
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 1
Abstract
Simple and effective high performance liquid chromatographic (HPLC) method was developed for estimation of Clindipine in drug free human drug free blank plasma. The internal standard used as Nifidipine (IS). The current method was used protein precipitating extraction of Clindipine from blank plasma. Separation was achieved on reversed-phase c18 column (25cm × 4.6mm, 5μ) and the detection was monitored by UV detector at 260 nm. The optimized mobile phase was used acetonitrile: 5mM potassium dihydrogen orthophosphate (pH 4.5), in the ratio of 60:40% v/v at a flow rate of 1.0 ml/min. This linearity was achieved in this method range of 10.0-125.0 ng/ml with regression coefficient range is 0.99. The present method is suitable in terms of precise, accurate and specific during the study. The simplicity of the method allows for application in laboratories that lack sophisticated analytical instruments such as LC-MS/MS or GC–MS/MS that are complicated, costly and time consuming rather than a simple HPLC–UV method. The present method was successfully applied for pharmacokinetic studies.
Authors and Affiliations
Selvadurai Muralidharan , Jaya raja Kumar , Sokkalingam Arumugam Dhanaraj
Keywords
Related Articles
- EP ID EP89158
- DOI -
- Views 207
- Downloads 0
Potent AChE enzyme inhibition activity of Zizyphus oxyphylla: A new source of antioxidant compounds
The purpose of this study was to assess the antioxidant potential and enzyme inhibition of various fractions of Zizyphus oxyphylla. The plant metabolites were extracted in methanol and partitioned with n-hexane, ch...
Anti-hyperglycemic and antinociceptive activity of methanol leaf and stem extract of Nypa fruticans wurmb
Nypa fruticans Wurmb. (Arecaceae) is a mangrove palm well-known for its traditional uses by the local practitioners against different ailments in southern regions of Bangladesh. However, the plant is yet to be scientific...
Evaluation of antioxidant potential and HPLC based identification of phenolics in Polygonum amplexicaule extract and its fractions
There is a growing interest for the plant-based medicines in pharmaceutical industry. Plant derived Antioxidants have gained huge importance regarding their medicinal value. The present study was designed to establ...
Preliminary phytochemical screening, Antibacterial potential and GC-MS analysis of two medicinal plant extracts
The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azad...
Effect of renin inhibition on adipokines in diabetic rats
Insulin resistance predicts development of type 2 diabetes mellitus (DM). Adipocytes release tumor Necrosis factor-alpha (TNF-α), and adiponectin. They modulate whole-body insulin sensitivity . The disturbance in t...