Simultaneous Estimation of Paracetamol and Tapentadol in Bulk and Pharmaceutical Formulation by Reverse Phase High Performance Liquid Chromatography (RP-HPLC)
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 6
Abstract
: Paracetamol and Tapentadol are both analgesic drugs. As there is no UV or HPLC method for the simultaneous estimation of Paracetamol and Tapentadol, a need was felt to develop the method for the analysis of both drugs simultaneously. This work concerns with the development and validation of a simple, specific and cost effective RP-HPLC method for simultaneous estimation of Paracetamol and Tapentadol in bulk and the developed method was applied to the pharmaceutical dosage form ie., Tapcynta. Chromatography was carried on Thermohypersil BDS C18 column with mobile phase comprising of Dihydrogen potassium phosphate buffer and Acetonitrile in the ratio of 50:50 v/v. The flow rate was adjusted to 0.9 ml/min with PDA detection at 218.6 nm. The retention times of Paracetamol and Tapentadol were found to be 4.8 min, 8.0 min respectively and other replicate standard system suitability parameters are within the limit and uniform. The different analytical parameters such as accuracy, precision, linearity, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined according to the International Conference on Harmonization (ICH) Q2B guidelines. The detector response was linear in the range of 88044x + 19214 mg/ml, 49855x + 36868 mg/ml for Paracetamol and Tapentadol respectively. The proposed method was successfully applied for the reliable quantification of active pharmaceuticals present in the commercial formulations.
Authors and Affiliations
Usharani Gundala , Chandrashekar Bonagiri , Devanna Nayakanti
Keywords
Related Articles
- EP ID EP116084
- DOI -
- Views 67
- Downloads 0
Phytochemical Evaluation And Pharmacological Screening Of Antiparkinson’s And Laxative Activities Of Hylocereus Undatus (White Pitaya) In Rodents
Objective: To evaluate the antiparkinson’s and constipation activity of Hylocereus undatus Haw. fruit extract in haloperidol induced parkinson’s disease and loperamide induced constipation in mice. Method: Parkinson’s di...
[b]COMBINED TITRI METRIC AND POLARI METRIC METHODS FOR DETERMINATION OF MALTOSE IN MALTING BARLEY[/b]
A Review on Current Trends of Nanotechnology for Cancer Therapy
Targeted drug delivery is an advanced method of delivering drugs to the patients in such a targeted sequences that increases the concentration of delivered drug to the targeted body part of interest only (organs/tissues/...
Effect of Sitagliptin in Combination with Glimepiride on Glycemic Control and Islet Cell Diameter/Proliferation in A Model of Type 2 Diabetic Rats
The aim of the present study was to investigate the effect of monotherapy with sitagliptin, or its combination with glimepiride in glycemic control, islet cell diameter and its proliferation in type 2 diabetic ra...
Studies on combined effect of Aeromonas hydrophila and cadmium on lipid peroxidation and antioxidant status in selected tissues of Indian freshwater major carp, Catla catla: role of silver nanoparticles
The present study was aimed to evaluate the effect of dual stressors cadmium (Cd) and Aeromonas hydrophila (AH) on pro-and antioxidant status in gills, kidneys and liver tissues of fishes, Catla catla treated wi...