SOMATIC EMBRYOGENESIS IN RUBUS CAESIUS L. SUSPENSION CULTURES
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2008, Vol 54, Issue 2
Abstract
In vitro cultures technologies include many applications: plants improvement, micropropagation, unconventional alternatives to exploit secondary metabolites. The regenerative potential of Rubus caesis L. was evaluated for the establishment of a propagation protocol. Not only the capacity of differentiation was tested, but also the regenerative potential. The initiation of in vitro cultures at Rubus sanctus Schreb was achieved from axillary buds, cultivated on different variants of Murashige-Skoog medium. The callus cultures obtained by axillary buds dedifferentiation were multiplicated on agar medium and liquid medium. The MS medium supplemented with 1mg/l benzylaminopurine and 0,1 mg/l IAA stimulated somatic embryogenesis at Rubus caesius L. suspension cultures ,whereas the MS medium supplemented with 1mg/ kinetine generated an intensive proliferative reaction and callus development. The regeneration of whole plants was obtained in three steps: callus initiation, suspension cultures initiation, somatic embryogenesis induction.
Authors and Affiliations
SMARANDA VÂNTU
Keywords
Related Articles
- EP ID EP140222
- DOI -
- Views 96
- Downloads 0
VARIANCE OF THE MINERAL CONTENT FROM DIFFERENT ORGANS OF TWO PISUM SATIVUM CULTIVARS
The paper presents the content in mineral elements from the organs of two Pisum sativum cultivars namely: Gerola and Oregon Sugar Pod. The Oregon Sugar Pod is a west European cultivar little known in Romania characterize...
NEW HIGH-RESOLUTION POLLEN RECORD FROM HAUTES-FAGNES, BELGIUM
THE OPTIMAL USAGE OF GROWING BIOSTIMULATORS FOR IN VITRO MULTIPLICATION PROCESS AT STEVIA REBAUDIANA (BERT.)
This paper presents a study about the „in vitro” multiplication of Stevia rebaudiana (Bert.) species and some conclusions about the influence of growing regulators’ concentrations in mixture for some biometrics character...
PROTECTED TAXA FROM THE BISTRIŢA RIVER BASIN BETWEEN PIATRA NEAMŢ AND BACĂU
The results presented in this article were obtained during a study made in 2005 and 2006 in the Bistriţa river inferior basin in the region between Piatra Neamţ and Bacău. As a result of this study were catalogued...
SPRUCE BARK POLYPHENOLIC EXTRACT INFLUENCES ON SAGE (SALVIA OFFICINALIS L.) INTERNAL STRUCTURE
The paper presents histo-anatomical aspects for sage (Salvia officinalis L.) species grown under the influence of spruce bark polyphenolic extract (SBPE). Sage was choose given the fact that it's well knew as an aromatic...