Thin Layer Chromatodraphy Method for the Determination of Ternary Mixture Containing Salbutamol Sulphate, Bromhexine Hydrochloride and Etofylline
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2010, Vol 2, Issue 3
Abstract
Simple, rapid, accurate, precise, reliable and economical thin layer chromatographic and spectrophotometric methods have been proposed for the resolution and determination of Salbutamol sulphate (SS), Brombexine hydrochloride (BH) and Etofylline (ET) in pure and pharmaceutical formulations respectively. The developed methods show best results in terms of resolution, linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) for standard laboratory mixtures of pure drugs and marketed formulations. The Rf value for SS was found to be 0.25, for BH Rf value was found to be 0.91 and for ET Rf value was found to be 0.71. The range for SS, BH and ET were found to be 1-35 µg mL-1, 4-40 µg mL- and 5-80 µg mL-1 respectively. The values of LOD were 0.245 µg mL-1, 0.521 µg mL-1 and 0.930 µg mL-1 and the values LOQ were 0.816 µg mL-1, 1.733 µg mL-1 and 3.071 µg mL-1 for SS, BH and ET respectively. The precision values were less then 2 % in terms of % relative standard deviation for the developed method. The common excipients and additives did not interfere in their determinations
Authors and Affiliations
H. N. Dave , R. C. Mashru , A. K. Patel
Keywords
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