NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG
Journal Title: Progress on Chemistry and Application of Chitin and its Derivatives - Year 2010, Vol 0, Issue
Abstract
With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.
Authors and Affiliations
Denis V. Kurek, Sergey A. Lopatin, Vladimir E. Tikhonov, Valery P. Varlamov
Keywords
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