New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme
Journal Title: Cumhuriyet Science journal - Year 2018, Vol 39, Issue 3
Abstract
In this study, we investigated adsorption behaviour of lysozyme on poly(hydroxyethylmethacrylate-expanded perlite) [P(Hema-Ep)]. For this purpose, we synthesized P(Hema-Ep) composite by bulk polymerization and it was characterized by SEM, FTIR and swelling ratio. The fresh chicken egg white lysozyme was used as a model protein, had an isoelectric point (pI) 10.7 and molecular weight (MW) 14 kDa. Adsorption capacity was found 51 mg g-1 at room temperature and in 0.1 M pH=8.0 phosphate buffer. Experiments on desorption and reusability were also performed. It appears that P(Hema-Ep) composites can be used ten times without lost of efficiency. Lysozyme purification, without causing any denaturation, can be performed from chicken egg white by using this new synthesized composite in a single step.
Authors and Affiliations
Birnur AKKAYA, Recep AKKAYA
Keywords
Related Articles
- EP ID EP488372
- DOI 10.17776/csj.423415
- Views 40
- Downloads 0
Evaluation of dosimetric impact of Flattening Filter-Free (FFF) Photon Beams in 3D conformal radiotherapy
Flattening Filter Free (FFF) photon beams have been frequently used in radiotherapy clinical practice. This study aimed to analyze the dosimetric quality of the FFF beams, as compared to the standard flattened beams (FB)...
Optimization of Molding Unused New Sand Composition by Mixture Design Method
In this study, various parameters which affect the green strength, gas permeability and shear strength of unused casting sands were investigated in order to improve the quality of casting sands used in Estaş casting foun...
DFT Study on Antioxidant Action Mechanisms of Naphthoquinone-Urazole Hybrids
The aim of this study is on the evaluation of the antioxidant activities of the investigated naphthoquinone-urazole hybrids by comparing our theoretical results with experimental results and on the elucidation the antiox...
An Adaptive Neuro-Fuzzy Inference System (ANFIS) of Radioactivity Levels in Hazar Lake
In this study, an Adaptive Neuro-Fuzzy Inference System (ANFIS) model is proposed for the determination of alpha radioactivity of Hazar Lake waters and for the prediction of its unknown values. The model parameters of th...
The Effects of Nisin and Natamycin on the Microbiological, Chemical and Sensorial Qualities of Meatballs
Meatballs are known to be one of the foodstuffs most sensitive to microbiological deterioration due to their physical and chemical properties. Meat and meat products are known to be the leading sources of food-related di...