New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme
Journal Title: Cumhuriyet Science journal - Year 2018, Vol 39, Issue 3
Abstract
In this study, we investigated adsorption behaviour of lysozyme on poly(hydroxyethylmethacrylate-expanded perlite) [P(Hema-Ep)]. For this purpose, we synthesized P(Hema-Ep) composite by bulk polymerization and it was characterized by SEM, FTIR and swelling ratio. The fresh chicken egg white lysozyme was used as a model protein, had an isoelectric point (pI) 10.7 and molecular weight (MW) 14 kDa. Adsorption capacity was found 51 mg g-1 at room temperature and in 0.1 M pH=8.0 phosphate buffer. Experiments on desorption and reusability were also performed. It appears that P(Hema-Ep) composites can be used ten times without lost of efficiency. Lysozyme purification, without causing any denaturation, can be performed from chicken egg white by using this new synthesized composite in a single step.
Authors and Affiliations
Birnur AKKAYA, Recep AKKAYA
Keywords
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- EP ID EP488372
- DOI 10.17776/csj.423415
- Views 58
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