New Validated Fluorescence Quenching Based Procedure for the Determination of Cilostazol and Clopidogrel in Bulk, Tablets and Biological Fluids, With Application of Stern-volmer Equation
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 12, Issue 6
Abstract
Aims: A very Sensitive, simple and selective Spectrofluorimetric method for the determination of Cilostazol or Clopidogrel through their reaction with fluorescein dye is developed. Determination was performed on raw material, tablet dosage form and extended for determination of cilostazol in spiked human plasma. Study Design: Quenching reaction between fluorescein dye and tertiary amine containing drugs. Place and Duration of the Study: This experiment was applied on analytical chemistry lab in faculty of pharmacy, analytical chemistry department, during the period (from 5 April to 10 May, 2016). Methodology: The formed ion-pair complex between the reagent and each drug results in decrease in the fluorescence intensity of the reagent, this decrease (fluorescence quenching ΔF) is directly proportional to concentration of both drugs at the range of (0.001-0.08 μg ml-1) and (0.01-0.50 μg ml-1) for cilostazol (CIL) and Clopidogrel (CLP), respectively. For cilostazol (limit of detection) LOD is (0.0003μg mL-1) and (limit of quantitation) LOQ is (0.0008 μg ml-1). For clopidogrel LOD is (0.0025 μg ml-1) and LOQ is (0.0076 μg ml-1). The affecting factors on fluorescence intensity of the product are carefully investigated and optimized. Using the optimized experimental conditions, the proposed quenching method is validated according to International Conference of Harmonization guidelines. The proposed method is perfectly applied to the analysis of commercial tablets containing each drug separately. Results: Statistical comparisons of the results with those of the reference methods illustrate good agreement and confirm that there was no significant difference in the accuracy and precision between the proposed and reference one for both drugs, respectively. The proposed method is extended for the in vitro determination of CIL in spiked human plasma as preliminary investigation; the mean recovery (n=5) was 99.80. Conclusion: Owing to the simplicity, quickness and cheapness of the present study, it can be applied for the routine quality control of the studied drug in its dosage forms.
Authors and Affiliations
F. Ibrahim, M. Sharaf El-Din, Heba Abd El-Aziz
Keywords
Related Articles
- EP ID EP342173
- DOI 10.9734/BJPR/2016/28329
- Views 72
- Downloads 0
Phytochemical and Nutritional Composition Analysis of Murraya koenigii Linn Leaves
Vegetable is an indispensable constituent of human diet that supplies the body with minerals, vitamins and certain hormone precursors, in addition to protein and energy. The phytochemical and proximate analyses of Murra...
Antioxidant Properties and HPLC Assay of Bioactive Polyphenols of the Ethanol Extract of Excoecaria agallocha Stem Bark Growing in Bangladesh
Aims: The ethanolic extract of Excoecaria agallocha stem bark was evaluated for antioxidant properties and quantification of bioactive polyphenols by HPLC-DAD. Methodology: ABTS assay, total antioxidant capacity, reducin...
Synthesis, Characterization and Evaluation of Biological Properties of Pyrazolo- and Oxazolo-pyridocarbazoles and Pyridoazacarbazoles as Potent Anti-microbial Agents
Aims: Fused carbazoles, azacarbazoles and quinolines are endowed with a wide array of pharmacological properties. Keeping these properties in the mind, the study was undertaken to seek structural modifications to generat...
Various Emerging Trends in Insulin Drug Delivery Systems
Lowering of blood glucose level in patients can be achieved by insulin therapy as it plays a key role in the control of hyperglycaemia for type 1 diabetes. Insulin delivery systems that are currently available include sy...
A Comparison of the Humoral Immune Response Induced by a Recombinant Human Protein in Wild Type Mice and in Transgenic Mice Expressing the Protein
Aim: The aim of this work was to investigate the correlation between anti drug antibody (ADA) induction and how different manufacturing processes of biopharmaceuticals affect the immunogenicity of the protein. This was d...