Ninhydrin Positive Eggshell Surface Proteins and Water Soluble Proteins of Six Domesticated Birds can Become Possible Tools to Assess Taxonomic as Well Genetic Diversity
Journal Title: International Journal of Zoological Investigations - Year 2018, Vol 4, Issue 1
Abstract
Avian eggshell is compartmentalized and has structural stratification like cuticle and matrix. Of these layers outer most layer is studied in this work to understand the nature of profiles generated by ninhydrin positive proteins. Eggs of six species of domesticated birds of the order Galliformes categorized under five different families were used for analysis. All the six egg species responded to ninhydrin producing Ruhemann’s purple colour of varied profiles, indicated the taxonomic family-wise difference in profiles suggest the possibility of using this technique for taxonomic considerations. The SDS-PAGE profile of water soluble proteins too showed greater variations both in number and molecular weight in five species of birds but such proteins were totally missing in Anas platyrhynchus, primarily a domesticated water bird. Total quantity of proteins estimated by Lowry’s method too revealed quantitative difference among the six species. The significance and possible utility of these findings are presented in study.
Authors and Affiliations
H. Channaveerappa
Keywords
Related Articles
- EP ID EP451479
- DOI -
- Views 164
- Downloads 0
Genetic Variation in Xeric Habitats of Triturus vittatus vittatus (Urodela) Using Mitochondrial DNA of 12S and16S, and Nuclear Gene, Rhodopsin, on the Southern Border of its Distribution
The present study examined the genetic variations of the newt (Triturus vittatus vittatus) (Urodela) from different breeding sites on thesouthern border of its distribution using mitochondrial DNA of 12S and 16S, and nuc...
Concept of Sustainable Bioremediation for Perpetual Control of Fish Diseases Caused by Watermoulds and Bacteria
The parasites and diseases constitute one of the important factors in regulating the fish population in Pisciculture. To increase fish production, it is necessary that the various contributory factors for mortality of fi...
Sublethal Toxic Effects of Bisphenol A on Oxygen Consumption, Haematological and Histological Parameters in the Cichlid Fish, Pseudetroplus maculatus (Bloch, 1795)
The cichlid fish Pseudetroplus maculatus was used to evaluate the sublethal toxic effects of estrogenic environmental contaminant, bisphenol A. Fish were exposed to sublethal concentration (648 μg/L) of bisphenol A for s...
Hypophysectomy Induced Histological Changes in Corpuscles of Stannius of a Freshwater Catfish, Heteropneustes fossilis
Freshwater catfish, Heteropneustes fossilis were collected and acclimatized. After acclimation they were divided into two groups A and B. Fishes from group A served as control whereas fishes from group B were hypophysect...
Drosophila as a Powerful Modern Tool for Teaching Concepts in Biology and Biomedical Research
Drosophila is a potential teaching tool to help students in understanding core concepts in biology. Flies have gained significant importance as one of the forefront model organism for research in different areas of Biolo...