Non-Structured Kinetic Model of Aspergillus niger Growth and Substrate Uptake in a Batch Submerged Culture
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 9
Abstract
Aims: To investigate cell growth profile of Aspergillus niger in a batch submerged culture medium and then evaluation of cell kinetic behavior using some different non-structured kinetic models. Methodology: Experiments of cell growth and substrate utilization were conducted in batch submerged cultures with identified medium composition. Fitness assessment of experimental data on the cell growth and glucose consumption by models was performed using the curve-fitting tool in Mat Lab software. This report is the first in the kinetic investigation of Aspergillus niger PTCC 5010 with the studied models. This work was performed in Department of Chemical Engineering, Islamic Azad University, Qaemshahr Branch between April 2013 to September 2013. Results: Based on the obtained results; Moser kinetic model with R2 equal to 0.913 and Gompertz kinetic model with R2 equal to 0.949 were the best fitted models to describe the growth behavior of Aspergillus niger PTCC 5010 in the applied culture condition. Maximum specific cell growth rate with Moser and Gompertz kinetic models were 0.024 and 0.003h-1, respectively. Other kinetic constants for all studied models were also determined at the applied culture conditions. The consistency of the experimental data with Monod, Verhulst and Contois kinetic models wasn't in an acceptable range. Conclusion: In scale up biochemical projects by Aspergillus niger PTCC 5010 for some industrial products, Moser and Gompertz kinetic models are able to demonstrate cell growth behavior and its substrate uptake profile. In continuous processes, dilution rate could be determined based on obtained maximum specific cell growth rate equal to 0.024 h-1.
Authors and Affiliations
Fatemeh Ardestani, Roxana Kasebkar
Keywords
Related Articles
- EP ID EP237720
- DOI 10.9734/BBJ/2014/11472
- Views 113
- Downloads 0
Antibiosis In vitro of Trichoderma Strains Metabolic Extract on Mycelial Growth and Reproductive Capacity of Fusarium oxysporum Isolated from Pepper Plants (Capsicum annuum L.)
Aims: To inhibit the mycelia growth inhibition and reproductive capacity of important phyto-pathogen fungus: Fusarium oxysporum by cell-extracts from submerged cultures of Trichoderma. Study Design: A complete randomized...
Production and Partial Characterization of Pectinase from Snail (Archachatina marginata) Gut Isolates
Aim: To identify isolates for industrial production of pectinase. Methodology: The isolates were screened for pectinolytic activity using pectin as substrate. Enzyme activity was expressed as mg of glucose equivalent rel...
Biotechnological Application of Cassava-Degrading Fungal (CDF) Amylase in Broiler Feed Formulation
In this study, an attempt was made to substitute maize (Zea mays L.) content of broiler starter feed with ground cassava (Manihot esculenta Crantz) peels enzymatically improved with amylase-producing fungi with a view to...
Effect of Gas Flaring on the Phytochemical and Nutritional Composition of Treculia africana and Vigna subterranean
Aims: The effect of petroleum exploration and exploitation activities on the phytochemical and nutritional composition of African breadfruit (Treculia africana) and Bambara groundnuts (Vigna subterranean) harvested from...
Establishment of Callus Derived from Jatropha curcas L. Petiole Explants and Phytochemical Screening
Aims: The focus of this study was to assess the production of secondary metabolites of callus derived from Jatropha curcas L. petiole explants. Study Design: Laboratory experimental tests; Tissue culture, Lyophilisation,...