Effects of Exogenous ABA on Contents of Lycopene and Endogenous Hormone in Tomato Pericarp
Journal Title: Biotechnology Journal International - Year 2016, Vol 16, Issue 1
Abstract
Aims: This research studied the regulatory effect of ABA (Abscisic acid) on the contents of lycopene and endogenous hperipheral pericarp of tomato fruit during ripening, and provided an effective measure for improving the quality of tomato fruits. Study Design: When the second cluster of toormone in mato fruits reached green mature stage, fruits were labeled and sprayed ABA (100 mg•L-1) over fruit surface, with spaying water as control, avoiding polluting the leaves. After treatment for 0, 4, 8, 12, 16, 20d, 10 fruits were sampled and dissected, peripheral pericarp for measuring the contents of lycopene, GA3 (Gibberellic acid), ABA, ZT (Zeatin) and ETH (Ethylene). Place and Duration of Study: Horticultural College, between February 2014 and March 2015. Methodology: The contents of lycopene, GA3, ABA and ZT were determined using HPLC (High Performance Liquid Chromatography). The content of ETH was determined using GC (Gas Chromatography). Results: The effect of exogenous ABA on the lycopene content in peripheral pericarp of tomato fruits was firstly inhibited then promoted during fruit mature period. Exogenous ABA decreased the content of endogenous GA3, but the endogenous ABA content was inhibited firstly, and then promoted, inhibited finally, and promoted the content decrease of endogenous ZT. The peak value was appeared early, but a little effect on the amount of ETH release. Conclusion: This work proved the feasibility of elevating of lycopene levels in tomato fruit by the application of exogenous ABA. ABA promoted the accumulation of lycopene by the decreasing of endogenous GA3, ZT and ABA contents, and by releasing peak appear of endogenous ETH.
Authors and Affiliations
Baoli Zhou, Yang Yu, Qian Weng
Keywords
Related Articles
- EP ID EP238019
- DOI 10.9734/BBJ/2016/28767
- Views 131
- Downloads 0
Isolation and 16S rRNA-Based Identification of Benomyl-Degrading Bacteria
Laboratory experiments were conducted to isolate and identify Benlate- (Benomyl) degrading microorganisms from two soil types collected from different locations in Khartoum State, Sudan. Benomyl degradation was studied a...
Phytochemical and Antimicrobial Screening of Root Extracts of Dacryodes edulis
Aims: To screen extracts of Dacryodes edulis phytochemically. To assay the said extracts for antimicrobial activity. To validate or otherwise claims by traditional medicine practitioners on Dacryodes edulis. Place and Du...
Changes in Microbial Population of Palm Oil Mill Effluent Polluted Soil Amended with Chicken Droppings and Cow Dung
Aim of Study: To assess changes in microbial population in palm oil mill effluent (POME) polluted soil amended with chicken droppings and cow dung. Study Design: 32 plots measuring 4 m2 were mapped out in a randomized c...
DNA Sequence Characteristics and Phylogenetics of Three Oligonucleotides Markers on Clariid Species
Aims: The aim of this study is to express the profiles of three oligonucleotide markers corresponded to reproductive genes that may be different between the two Clariid species (Clarias gariepinus and Heterobranchus bido...
Effect of Argon Laser Curing on the Shear Bond Strength of Composite Resin Restorative Material
Aim: The aim of this in vitro study was to analyze the shear bond strength of composite resin using two different curing light sources: halogen light (control group) for 20 or 40s, and Argon laser (test group) for 10 or...