Novel Technique for Rapid Screening of Malarial Parasite by Toluidine Blue Staining
Journal Title: Indian Journal of Pathology: Research and Practice - Year 2018, Vol 7, Issue 10
Abstract
Introduction: Malaria is a tropical disease causing millions of death worldwide. Early detection and management prevents complications and mortality. Definitive diagnosis depends on demonstration of malaria in blood smears. Aims & objectives: In this study we intend to evaluate Toluidine blue utility and efficacy in screening & species identification of malaria parasite in peripheral smear. Materials and methods: In 134 patients with clinical suspicion of malaria, peripheral smears were prepared and were processed with both conventional Leishman method and Toluidine blue method simultaneously. Results: Out of 134 smears, 51 were positive for malaria with male preponderance and age incidence being more between 41-50 yrs. Predominant species was Plasmodium vivax (76.47%). Leishman stain showed decreased sensitivity (84.3%) and negative predictive value (91.2%) when compared to Toluidine blue stain. Conclusion: Toluidine blue staining method helps to identify parasites even at low power objective allowing screening more areas in shorter time. It is specifically useful in low parasite index cases and in species identification
Authors and Affiliations
Bheemaraju Venkata Vydehi
Keywords
Related Articles
- EP ID EP529594
- DOI 10.21088/ijprp.2278.148X.71018.1
- Views 49
- Downloads 0
Clinicopathological Evaluation of Uterine Sarcomas: An Institutional Experience
Introduction: Uterine sarcomas are highly malignant tumors accounting for 3-7% of all uterine malignancies. According to the World Health Organization, uterine sarcomas consist of two main categories: mesenchymal tumours...
Is Cervical Cancer Incidence Decreasing in the Urban Population of India with Developing Healthcare, Socio Demographic Progress and Awareness Programmes?
Context: Last few decades saw rapid decline of cervical cancer worldwide. However, 75% of the new cases are reported to occur in developing countries due lack of awareness among the female population. Aims: This study w...
Histopathological Pattern Analysis in Non Neoplastic Skin Biopsies: A Prospective Study of 142 Cases
Background: Non neoplastic skin lesions form the majority of morbidity from skin diseases, however majority of them are often diagnosed as non specific dermatitis. The objective of this study was to analyse and classify...
A Clinicopathological Study and Immunohistochemical Expression of p53 in Ovarian Tumors
Background: Ovarian tumours are heterogenous neoplasms with a varied clinical, morphological and histological features. p53 overexpression has been associated with poor prognosis, poor overall survival and altered sensit...
Correlative Study of Elisa and Nuclic Acid Test in the Diagnosis of Transfusion Transmittable Viral Diseases
Introduction: Blood transfusions are one of the major life saving procedures carried out to replace the host blood with that of donor to maintain the circulatory volume of blood. As most of the pathogenic microbes gets t...