Observation of Esterase-Like-Albumin Activity during N’- Nitrosodimethyl amine Induced Hepatic Fibrosis in a Mammalian Model
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2012, Vol 5, Issue 1
Abstract
Aim: Hepatic fibrosis (HF) is characterized by irregular growth and amassing of fibrous scar tissues in the liver causing weakened hepatocytes metabolism and protein level alterations, including albumin. Albumin with M r~68-70 kDa is unglycosylated soluble plasma protein with various biological roles. In this study, we demonstrate ‘esterase-like activity’ of albumin during NDMA-induced HF in rats. Material and Methods: In rats, HF was induced by weekly i.p. injections of NDMA in doses of 10 mg/ kg b.wt. Sera of controls (untreated) and treated rats were processed for biochemical tests, electrophoretic profiling and in-gel esterase activity localization using α, β-naphthyl acetates. H&E staining of liver sections (~ 5 μm) was done to confirm induction of HF. Results: NDMA satisfactorily induces hepatic fibrosis within 21 days which is also evident by significant increase in SALP, SGOT, SGPT and bilirubin levels in rats. ‘Esterase-like activity’ of albumin detected in animal sera remains stable throughout the course of treatment irrespective of other biochemical changes. Conclusion: During pathogenesis of HF, formation of stable esterase-albumin complex may have some important role and hence, prior recommending the use of albumin as diagnostic marker we propose further investigations to elucidate the mechanism of its formation.
Authors and Affiliations
Areeba Ahmad| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology, Veena Maheshwari| Department of Pathology, J.N. Medical College, and Hospital, Aligarh Muslim University, Aligarh-202 002 (U.P), India, Arif Ahmad| Department of Biosciences, Jamia Millia Islamia, New Delhi-110 025, Rashid Saleem| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology, Riaz Ahmad| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology
Keywords
Related Articles
- EP ID EP8846
- DOI 10.3889/MJMS.1857-5773.2012.0214
- Views 261
- Downloads 15
Macedonian Medical Doctor of Philosophy (PhD) Theses Defended in 2012
We present English abstracts of PhD theses defended in 2012 at the Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia. English summaries are published as they are translated by aut...
Current Scientific Impact of the Institutes, Faculty of Medicine, University “Ss Kiril and Metodijâ€, Skopje, Republic of Macedonia
Background. Institutes at the Faculty of Medicine in the Republic of Macedonia are constitutive parts with integrated educational, scientific and health care protection activities. They are included in all educational...
Acinetobacter spp. – A Serious Enemy Threatening Hospitals Worldwide
Acinetobacter is gram-negative, strictly aerobic bacteria. It is a heterogeneous group of organisms that is ubiquitous, widely distributed in nature. Surviving in hospital environment (they are able to survive on dry p...
Different Ankle Brachial Index Levels in Asymptomatic Hemodialysis Patients
Resting ankle brachial systolic pressure index (ABI) level of 0.90 is 95% sensitive in detecting angiogram-positive peripheral arterial disease (PAD) and that falsely elevated pressures or incompressible arteries at th...
Occult Glove Perforation During Adult Elective Orthopaedic Surgery
Aim: This prospective study was conducted in Rustaq Regional referral hospital, Oman from 01-3-2004 to 31-2-2007 to study the frequency of occult glove perforation in adult elective orthopaedic operations and to determ...