Obtaining cultural antigen for diagnostics equine infectious anemia in the DPR test
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 31, Issue
Abstract
Introduction. One of the most important challenge in horse breeding is equine infectious anemia (EIA) that constantly registers in many countries with the developed breeding horses. Diagnostics of EІA is a basic chain in the system of preventive measures. The basic serum method of diagnostics is a reaction of diffuse precipitation (Coggins test).The goal of the work. To construct the diagnostic test-kit for gel diffuse precipitation reaction (DPR) on the basis of group specific antigen received from concentrate cultural virus strain “З-ВІЕВ-К” and monitoring researches of horse in the Western region of Ukraine on the presence of antibodies to the EIA virus.Materials and methods. The strain of EIA virus “З-ВІЕВ-К” was cultivated on primary and subcultures of horse embryos organs and passaged culture of trachea of calf (ТC). The virus in cultural liquid was determined by DPR, using “A set for diagnostics of equine infectious anemia in the reaction of diffuse precipitation” (Shcholkov biofactory). Concentrating of the cultural viral liquid, where virus cytopathic effect was detected, conducted using poliethylenglycol (PEG 6000) by a dialysis at a room temperature during a day.Results of research and discussion. A precipitation antigen was approved after researches of horses blood sera of the Western Polisya on the presence of specific antibodies to the virus of EIA. The serological researches of the collected samples conducted in parallel using commercial test kit for EIA diagnostics. As a result of the studies with obtained antigen it was set 3% positive samples of the general number of tested horses. The same results were revealed in researches using DPR of control test kit for EIA.Conclusions and prospects for further research. We obtained precipitating antigen of cultural EIA virus and developed test-kit “Antigen specific serum for EIA diagnostics in the agar gel diffuse precipitation reaction”. For the improvement of epizootic situation on EIA it is necessary to conduct the permanent diagnostic researches with the maximal scope of horse population and implementation of preventive measures for horse farm recovery.
Authors and Affiliations
Pavel Kryvoshyja, L. Kot, Oleg Rud
Keywords
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- EP ID EP266062
- DOI 10.31073/vet_biotech31-08
- Views 152
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