Obtaining transgenic papaya plants var. Maradol Roja with an antisense ACC oxidase gene
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 2
Abstract
Papaya is a crop of economic importance for tropical countries. However, post-harvesting losses of Maradol Roja cultivar, the most important in Central America, have been up to 80%, due to a lack of proper infrastructure and qualified personnel at the rural areas and mainly because of the quick ripening of the fruits. The ripening process in climacteric fruits like papaya is regulated by ethylene levels. By reducing the genetic expression of the key enzymes which participate in the biosynthesis of this phytohormone, a decrease of the expression could be obtained. With the purpose of obtaining transgenic plants with delayed fruit ripening, accox1 gene encoding for the 1-aminoiciclopropeno-1-carboxylate (ACC) oxidase of the Maradol Roja variety was isolated and a binary vector for gene gun mediated transformation constructed with this gene in antisense orientation. In vitro transgenic lines, verified by PCR, were obtained.
Authors and Affiliations
Orelvis Portal, Edrey A. Rodríguez, Jorge Gallardo, Neyda Bacallao, Ana L. Darías, Rafael Gómez, Elio Jiménez
Keywords
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