ONTOGENETIC CHANGES IN THE ACTIVITY OF TRYPSIN-LIKE ENZYMES IN INDIVIDUALS OF THE LABORATORY POPULATION OF DROSOPHILA MELANOGASTER AND DROSOPHILA VIRILIS
Journal Title: Вісник Одеського національного університету. Біологія - Year 2019, Vol 24, Issue 1
Abstract
Introduction. The ontogenetic variation of body mass index and activity of trypsin-like enzymes in individuals of laboratory populations of two species of fruit fly: Drosophila melanogaster and Drosophila virilis were studied. The aim of the work was to investigate the level of fluctuation of trypsin-peptide hydrolase activity at different stages of ontogenesis in separate individuals of the laboratory populations of Drosophila virilis and Drosophila melanogaster and to compare the activity indicators of the studied species. Methods. The material of the study was the 3-day-old larvae, pupae and adults of two Drosophila species that were cultivated under standard conditions. In individually prepared homogenates, the total activity of trypsin-like enzymes was determined using the specific substrate N-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA). The enzymatic hydrolysis of BAPNA was carried out in an alkaline medium in the presence of peptide hydrolases. The dynamics of optical density (ΔE) was determined at λ = 382.5 nm. The calculation of the relative activity of the enzymes was carried out on the basis of the extinction values obtained. The specific activity was found by the formula. The obtained primary data were processed statistically and presented in the form of variation curves and histograms. Results and conclusion. The variation curves of the body mass for larvae, pupae and imago were built by the data of separate individuals. The ontogenetic changes of the individual relative and specific activity of trypsin-like enzymes at the population level were determined. The average body mass index and activity of trypsin-like enzymes for each stage of post-embryonic development of Drosophila were calculated. The ranges of variation of the studied parameters under the stationary conditions of Drosophila cultivation were determined. It was determined that the highest indices of the body weight of individuals and their proteolytic activity (by the data of species melanogaster) coincide with the larval phase of the development. Possible causes of individual differences in the expression of the activity of trypsin-like enzymes in Drosophila are discussed.
Authors and Affiliations
A. M. Andrievskii, J. Yu. Podzolkova, I. L. Ryzhko, S. L. Pasternak
Keywords
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- EP ID EP662425
- DOI 10.18524/2077-1746.2019.1(44).167884
- Views 53
- Downloads 0
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