Optimization and Production of Phytase by Bacillus subtilis and its Agricultural Application
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2017, Vol 5, Issue 5
Abstract
In an attempt to explore extracellular phytase producing Bacteria from soil, the culture B-7 isolate was selected on the basis of formation of the clear zone on PSM plate and by assaying fermented broth. The isolate B-7 identified as Bacillus subtilis through morphological, cultural and Biochemical characterization as per Bergey’s Bacteriological analysis. As the fermentation, the process is significantly influenced by various components present in the medium and its concentration. Hence, the isolate was tested in varied concentrations of components as per Plackett-Burman method. The maximum production of phytase was observed at 72 hours of incubation at RT and at pH 6.5. The most suitable medium is wheat bran extract medium shows 81.30 U/ml. the study also shows that the di-potassium hydrogen phosphate has a negative effect on phytase production and wheat bran shows strong positive effect. Finally, the application of Phytase was carried out by an In-vitro and in-situ method on Maize, Sorghum, and wheat. In the In-vitro method, maize is the best-supported Plant as Shoot is concerned. In Insitu method Maize, Sorghum, and wheat give 12mm, 18mm, 7.5mm root length, and 13mm, 10mm, 11mm shoot length after 12 days.
Authors and Affiliations
S. P. Kusale, Y. C. Attar
Keywords
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