OPTIMIZATION OF CHITOOLIGOSACCHARIDES PRODUCTION USING IMMOBILIZED CHITOSANASE
Journal Title: PARIPEX-Indian Journal of Research - Year 2017, Vol 6, Issue 7
Abstract
Chitooligosaccharides (COS) are the pharmaceutically important product derived from chitosan using chitosanase. Enzyme immobilization helps to reuse the enzyme to certain extent without compromising the quality of product. In this study, COS production was carried out using chitosanase immobilized on to 2% calcium alginate. Immobilized chitosanase shown maximum COS production at pH 9 and temperature 50º C. COS production was maximum at 10 h incubation time. Immobilized chitosanase beads were reused up to 3 cycles. Hence, COS could be produced using this immobilized chitosanase without affecting the quality of product.
Authors and Affiliations
G. Arunkumar, K. Bhanumathi
Keywords
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