Optimization of Chromatographic Criterion to Recover Indian White Shrimp (Fenneropenaeus indicus) Alkaline Phosphatase from Hepatopancreas
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 9
Abstract
Commercially important alkaline phosphatase can be recovered from the hepatopancreatic tissue of Indian white shrimp with optimum yield, activity, and purity by carefully designing the criterions of ion exchange chromatographic purification. The tissues were homogenized, clarified, and concentrated. Concentrated homogenate was purified with optimum yield, activity and purity by optimising criterions of chromatography such as pH and ionic strength of the binding buffer, and ionic gradient and the flow rate of the elution buffer in DEAE-cellulose column. The enzyme was optimally bound to the column at pH 8.4 and ionic strength 0.1 M NaCl at flow rate of 1mL/min, and eluted with high resolution at ionic gradient of 0.10-0.35 M NaCl in 25 min at a flow rate of 1.5mL/min, and hence is the optimum criterions of chromatographic purification.
Authors and Affiliations
Krishna Prasad Nooralabettu
Keywords
Related Articles
- EP ID EP237727
- DOI 10.9734/BBJ/2014/12289
- Views 94
- Downloads 0
Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)
Aims: The present study was conducted with a view to develop an efficient protocol for high frequency plant regeneration of Brassica campestris for further crop improvement program by biotechnological manipulation and to...
Exploration of Actinobacteria from Mangrove Ecosystems of Nizampatnam and Coringa for Antimicrobial Compounds and Industrial Enzymes
Aim: A study was made to examine the kinship between the seasonal distribution of actinobacteria and the physico-chemical properties of the mangrove sediments of Nizampatnam and Coringa located along the South East coast...
Toxicological Effects of Plastic Composted Soil on Nitrifying Bacteria
Aim: The aim of this study was to evaluate the toxicological effects of plastic composted soil on some nitrifying bacteria which are Nitrosomonas sp. and Nitrobacter sp. Methodology: Five plastic composted soil samples w...
Inhibitory Effects of 4T1 Breast Tumor Transplantation on Mouse Peripheral Blood Immune Cell Populations
Aims: One of serious threats to women's health is mammary cancer whose occurrence, development, and treatment are related to the body’s immunological circumstances. In addition, the cancer also imposes the some effects o...
African Cassava: Biotechnology and Molecular Breeding to the Rescue
Cassava is an important African food crop, where it is a staple to about 250 Million people. It is a household name in Nigeria, the world largest producer of the root crop. It is propagated from stem cuttings and well kn...