Optimization of Cultural Parameters for Cost Effective Production of Kojic Acid by Fungal species Isolated from Soil
Journal Title: Microbiology Research Journal International - Year 2015, Vol 7, Issue 5
Abstract
Two different species of Aspergillus are able to produce kojic acid at higher concentrations under static conditions in the present study. Various physicochemical parameters which influence the production of kojic acid are optimized by means of One-factor-at-a-time method. The other objective of this research is to examine the prospects of using novel substrates like Cassava, Ipomea batatas, Alocasia macrorrhiza tubers and rice bran, wheat bran for the production of value added products like kojic acid. The kojic acid concentration is quantitatively estimated by Bentley’s colorimetric method. It is observed that the maximum yield of kojic acid crystals, 22.5 g/L is obtained with the substrate Cassava and by the isolate Aspergillus flavus. The optimized physicochemical parameters are initial pH - 6.0, Time - 28d, Temperature - 28ºC, Substrate concentration – 100 g/L, Nitrogen source concentration-2 g/L, MgSO4.7H20-0.5 g/L and KH2PO4-2.0 g/L under static conditions. After the fermentation is completed, the fermented broth samples are subjected to crystallization and the isolated kojic acid crystals are examined by Fourier transform infrared spectroscopy and X-ray crystallographic methods. The FTIR spectrum of the sample kojic acid showed bands at functional groups at 3270.8 cm-1, 3179.43 cm-1 (-OH), 2925.17 cm-1, 2854.05 cm-1 (aliphatic-CH), 1660.59 cm-1 (cyclic-C=O), 1611.11 cm-1 (C=C), 1472.61 cm-1 (deformation of-CH2), 1074.04 cm-1 (cyclic C-O-C), 943.58 cm-1, 863.66 cm-1 and 775.65 cm-1 (1,4 α -disubstituted ring). The X-ray diffraction spectrum of the sample is similar to standard sample and reveals seven distinctive peaks appeared at 2θ angles 8º, 19.27º, 21.71º, 27.73º, 31.15º, 36.13º, 39.09º. The result confirms the presence of kojic acid. Antimicrobial activity of kojic acid is tested on some Gram positive and Gram negative bacteria. The culture Escherichia coli and Staphylococcus aureus show equal susceptibility to the antimicrobial compound kojic acid.
Authors and Affiliations
Kayitha Bala Durga Devi, Payala Vijayalakshmi, Vadlamani Shilpa, Bapatla Veerendra Kumar
Keywords
Related Articles
- EP ID EP354770
- DOI 10.9734/BMRJ/2015/15325
- Views 73
- Downloads 0
Effect of Woodchips on Bioremediation of Crude Oil-polluted Soil
Aims: To evaluate the effectiveness of woodchips in bioremediation of crude oil contaminated soil, phytotoxicity assay as an index of soil biological activities (germination index) using a selected agricultural seed (Vic...
Carbapenem-hydrolyzing Oxacillinase Genes in Clinical Isolates of Acinetobacter baumannii
Aims: We aimed in this study to detect the prevalence of carbapenem-hydrolyzing oxacillinase genes among Acinetobacter baumannii clinical isolates recovered from Assiut University Hospitals, Egypt. Methods: The antimicr...
Enhancement the Antibacterial Potential of the Biosynthesized Silver Nanoparticles Using Hydrophilic Polymers
Silver nanoparticles are well recognized for its antibacterial activity, especially against pathogenic bacteria. In the present study, silver nanoparticles (AgNPs) were biologically synthesized by using the aqueous leave...
Isolated Bacterial Uropathogens among Pregnant Women Diagnosed with Asymptomatic Bacteriuria in Harare, Zimbabwe
Aim: The aim of this study was to identify bacterial uropathogens responsible for asymptomatic bacteriuria among pregnant women registering for antenatal care at selected clinics in Harare, Zimbabwe. Study Design: A cros...
Patterns of Initial Drug Resistance of Mycobacterium tuberculosis Isolates from Kashmir Valley, India
Aims: We carried out this study to determine the patterns of initial drug resistance in Mycobacterium tuberculosis isolates and prevalence of MDR-TB among category-I pulmonary TB patients in Kashmir Valley. MDR-TB was de...