OPTIMIZATION OF CULTURE CONDITIONS OF STREPTOMYCES CARPATICUS (MTCC-11062) FOR THE PRODUCTION OF ANTIMICROBIAL COMPOUND
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To improve the antimicrobial compound productivity of Streptomyces carpaticus (MTCC-11062) by optimizing its physical and chemical parametersMethods: Streptomyces carpaticus (MTCC-11062) was isolated from Visakhapatnam sea coast of Bay of Bengal and was screened for its antimicrobial activity by using agar well diffusion method. To improve the production of antimicrobial compound the medium composition and physical parameters were optimized and its productivity was studied against Bacillus cereus (MTCC 430) Escherichia coli (MTCC 443), Candida albicans (MTCC 227) obtained from MTCC, Chandigarh, India.Results: Optimum growth of mycelium and antimicrobial compound production occurred at pH 7.2, agitation 180 rpm and temperature 300C with glucose 10g/L, soyabean meal 2.5g/L, K2HPO4 2g/L, MgSO4 1g/L, NaCl 7.5g/L and trace salts.Conclusion: The optimization of cultural conditions proposed in this paper has effetely improved the antimicrobial compound productivity of Streptomyces carpaticus (MTCC-11062).
Authors and Affiliations
M Bhavana, Vssl Prasad Talluri, K Shiva Kumar, S. V. Rajagopal
Keywords
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