Optimization of Diesel oil degrading Bacterial strains at various culture parameters
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 6
Abstract
Environmental pollution with petroleum has been recognized as one of the most serious current problems. Petroleum hydrocarbons are a combination of hydrocarbons obtained from reservoirs of crude petroleum. The petroleum hydrocarbons contain aliphatic hydrocarbons and aromatic hydrocarbons. The most common petroleum hydrocarbons polluting environment are the gasoline, diesel and fuel oils. Numerous microorganisms could use petroleum as only source of carbon as energy for metabolic activities, and these microorganisms are broadly present and distributed in nature. Bioremediation is the use of microorganism metabolism to remove pollutants. Bioremediation is the biological process involving living microorganisms to remove contaminants or pollutants from soil or water. It involves the use of microbes to detoxify and humiliate environmental contaminants. The present study is carried out by optimization of diesel oil degrading microbes from diesel oil polluted sites. Bacillus sp and Enterobacter hormaechei were isolated according to Bergey’s manual and by 16s rRNA gene sequence. Further the optimization was carried out for various culture parameters such as various hydrocarbon concentration, pH, temperature and for best carbon and nitrogen sources. The present study suggests that Bacillus sp and Enterobacter hormaechei were able to degrade diesel oil at pH 7, 300 C and preliminary hydrocarbon concentration of 1%.
Authors and Affiliations
T. Sivagamasundari| Assistant Professor, Department of Microbiology and Biotechnology, N.M.S.S. Vellaichamy Nadar College, Madurai, India, N. Jayakumar| Assistant Professor, Department of Microbiology, Government Arts College, Surandai, India
Keywords
Related Articles
- EP ID EP16050
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2840-2
- Views 334
- Downloads 39
Cancer: An Overview
Cancer is the second leading causes of deaths in western countries after heart disease. In US alone more than one million people are diagnosed with cancer annually and more than half result in death. Cancer is a medica...
Evaluation of anti-obesity activity of Centratherum anthelminticum in obese rats
The effect of Ethanolic extracts of Centratherum anthelminticum on adiposity index serum levels of liver enzyme in obese rats investigated. twenty mature (180-210gm body weight) Swiss albino rats either sex randomly di...
ELECTRONIC TRIAL MASTER FILE (eTMF) - AN INDISPENSABLE TOOL THAT COLLECTS AND FILES ESSENTIAL DOCUMENTS OF A CLINICAL TRIAL: A REVIEW
Every organization involved in clinical trials in the BioPharma industry maintains a trial master file comprised of thousands of pages of regulatory documents required for each clinical trial. Managing thousands of clin...
Inhibition of Pro-Inflammatory Cytokine TNF-α by Boerhaavia diffusa (L.) in Liposaccharide stimulated Human THP-1 cells
The production and release of proinflammatory cytokines such as Tumor necrotic factor-α (TNF- α), Interleukin-1β and Iinterleukin-6 from monocytes/macrophages play critical role in the pathogenesis of chronic inflam...
Evaluation of Synergistic Anti-microbial efficacy of plant extracts and their formulation as Topical Agents
The objective of the present study was to evaluate the antimicrobial activity of Cassia fistula, Ficusreligiosa, Milletiapinnataand Wendlandiathyrsoideaand to check the synergistic efficacy of these extracts when combi...