OPTIMIZATION OF GROWTH CONDITIONS AND DEVELOPMENT OF A LABORATORY MODEL RACE WAY POND FOR CULTIVATION OF Spirulina platensis IN ANAEROBICALLY DIGESTED CASSAVA SAGO FACTORY EFFLUENT
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 37
Abstract
Sago effluent pose a serious environment pollution, if discharged on both soil and water bodies without proper treatment. In this study, we attempted cultivation of Spirulina platensis using anaerobically treated sago effluent diluted with water at different dilution levels viz., 80:20, 60:40, 50:50, 40:60, 20:80 and 100% (Undiluted) supplemented with different concentrations of NaHCO3 and NaNO3 sources as carbon and nitrogen respectively, based on Zarrouck’s broth composition. Results showed that the most suitable dilution of sago effluent with water for maximum growth of Spirulina platensis was 80:20 dilution with addition of NaHCO3 at 0.2 M (16.8 g L-1) and NaNO3 at 0.03 M (2.5g L-1) concentrations. The lab model raceway pond (65 L working volume) was designed to study the feasibility of Spirulina cultivation in the anaerobically digested sago effluent, with the optimized dilution and nutrient supplementation levels. It was found that Spirulina platensis was able to produce a biomass of 0.4g L-1 on dry weight basis with in a period of 18 days. The population of Spirulina platensis was 5 ×104 cells mL-1 with OD value of 0.753 and protein content 57%. The BOD, COD, TSS, TDS and organic carbon content of sago effluent were also significantly reduced indicating that sago effluent is further purified by Spirulina cultivation, which also serves as the single cell protein for farm animals.
Authors and Affiliations
KARTHIK MANICKAM
Keywords
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