Optimization of Immobilized Aldose Reductase Isolated from Bovine Liver

Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 2

Abstract

Objectives: Isolation of enzymes and experiments on them require great effort and cost and are time-consuming. Therefore, it is important to extend the usability of the enzymes by immobilizing them. In this study our purpose was to immobilize the enzyme aldose reductase (AR) and to optimize the experimental conditions of the immobilized AR and compare them to those of free AR. Materials and Methods: AR was isolated from bovine liver and the enzyme immobilized in photographic gelatin by cross-linking with glutaraldehyde. Then the optimum conditions for free and immobilized AR in terms of pH, temperature, and storage were characterized by determining the enzyme activity. Results: Following immobilization, the optimum pH and temperature levels for free AR, which were pH 7.0 and 60°C, slightly altered to pH 7.5 and 50°C. The enzyme activity of the immobilized AR was maintained at about 65% after reusing 15 times. Moreover, immobilized AR maintained 95% of its original activity after 20 days of storage at 4°C, while the retained activity of the free AR was 85% of the original. Conclusion: Our experiments indicated that the conditions that affect enzyme activity might alter following immobilization. Once the optimum experimental conditions are fixed, the immobilized AR can be stored and reused with efficiency higher than that of free AR. Moreover, this study provides an insight into the advantages of using immobilized AR in enzyme assays rather than free AR.

Authors and Affiliations

Marya Vakıl NASLIYAN, Sidar BEREKETOĞLU, Özlem YILDIRIM

Keywords

Related Articles

A Novel Genotyping Method for Detection of the Muscarinic Receptor M1 Gene rs2067477 Polymorphism and Its Genotype/Allele Frequencies in a Turkish Population

Objectives: Gene variation in the cholinergic muscarinic receptor 1 (CHRM1) has potential to become a candidate biomarker in the development of several disorders as well as drug response. In this study, a novel polymeras...

Essential Oil and Fatty Acid Composition of Endemic Gypsophila laricina Schreb. from Turkey

Objectives: Gypsophila species have very high medicinal and commercial importance and contain interesting natural substances. However, there is no report on the essential oil or fatty acid composition of any Gypsophila s...

A Validated Reverse Phase-Ultra-Performance Liquid Chromatography Method for the Determination of Gemifloxacin Mesylate in Bulk and its Pharmaceutical Preparation

Objectives: Gemifloxacin Mesylate is a fourth generation fluoroquinolone antibacterial agent. A simple, accurate, and precise reversed phase (RP)-ultra performance liquid chromatography (UPLC) method was developed and va...

Global DNA Hypomethylation and Rassf1a and c-myc Promoter Hypermethylation in Rat Kidney Cells after Bisphenol A Exposure

Objectives: Bisphenol A (BPA) is a synthetic monomer used in the production of polycarbonate and an environmental contaminant with endocrine disrupting properties. BPA release from plastic carriers is thought to cause hi...

Preclinical Evaluation of the Haematinic Activity of an Oral Indiffusible Mixture of Tamarindus indica L. Leaf Extract

Objectives: Tamarindus indica L. is known to be a multipurpose traditional plant in India. It is used to treat some bacterial infections, parasitic infestations, constipation, and inflammation. It is also used as a blood...

Download PDF file
  • EP ID EP684683
  • DOI 10.4274/tjps.galenos.2018.81894
  • Views 160
  • Downloads 0

How To Cite

Marya Vakıl NASLIYAN, Sidar BEREKETOĞLU, Özlem YILDIRIM (2019). Optimization of Immobilized Aldose Reductase Isolated from Bovine Liver. Turkish Journal of Pharmaceutical Sciences, 16(2), -. https://europub.co.uk/articles/-A-684683