OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS
Journal Title: Journal of Engineering Studies and Research - Year 2017, Vol 23, Issue 1
Abstract
This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobile phase pH and the column temperature. The best results in terms of separation were obtained using a mobile phase composed of acetonitrile and ammonium formate (150 mM pH 3) 85:15 v:v.
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- EP ID EP659798
- DOI 10.29081/jesr.v23i1.261
- Views 110
- Downloads 0
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