Optimization of the treatment and extraction procedures for Cetellaasiatica L. Urban.
Journal Title: International Journal of Pharmaceutical Science Invention - Year 2017, Vol 6, Issue 6
Abstract
The study is to research and apply enzyme Viscozyme supported for increase the possibility of constituents from Centellaasiatica (CA) that has biological effectiveness. The results show that the Centellaasiatica extract (CAE) yield is higher twice when comparing with the classical extractmethod (only using ethanol solvent). The detailed results: Surveying pre-treatment conditions of Viscozyme reached to the highest effectiveness at the concentration 2%, at 450C, in 90 minutes with Centella/ enzyme: 1/8. After CA treated with by enzyme will be extracted twice with ethanol 80%, in 60 minutes and at 400C with Centella/enzyme: 1/9 (w/v) whichreached 46,22%. The optimization conducted RSM-CCD Minitab 16 software with the results: CA treated withViscozyme 2,5%, at 430C. Then, extracted three times by ethanol 75% with mixture ratio ratio of solid to solvent (1/9 w/v), the optimized CAE yield is 49,21%, The method of optimization is used Minitab 16 that helped to reducethe extracted ethanol concentration(5%), temperature at 50C, also reducing the extracted time concentration (5,5 hours) when comparing with the old method. The extraction of asiaticoside from optimized CAE process (RMT) reached 37,91 mg/ml, higher 2,32 times when comparing with the classical extract method (RM1) only reached 16,31 mg/ml. The study has applied enzyme in the process which helped the time of the extracted process shorter, reducing temperature and the concentration of the extracted solvent, increasing the content of extracted asiaticoside.
Authors and Affiliations
Nguyen Thai Binh, Huynh Ngoc Oanh
Keywords
Related Articles
- EP ID EP406438
- DOI -
- Views 111
- Downloads 0
In Vitro Antimicrobial Activity Screening of Maclura pomifera Fruits Against Wide Range of Microorganisms
The use of plants as medicine has been practiced from ancient time in World. In developing country, traditional herbs have critical significance for disease treatment and they aid to detect novel antibiotics.Maclurapomif...
Antifungal Activity Of Coccinia Indica Extracts Against Fungal Pathogens
In recent years there has been renewed interest in screening plants for novel biologically active products particularly to combat ailments, which have defied synthetics and antibiotics. Medicinal plants offer a vast sour...
MRI Contrast Agents: Developments, Challenges, and Future Trends
Contrast agents in medicine are chemical materials used to improve tissue and fluid contrast in the body during medical imaging. It is mainly used in improving the visibility of blood vessels and the gastrointestinal tra...
Etiology and Antimicrobial Sensitivity Profile of the Microorganism Associated With Urinary Tract Infections
This study proposes to meet the most common aetiologic agents in urinary tract infection and the antimicrobial susceptibility pattern. We analyzed all bacteriological examinations of urine recorded in Central Laboratory...
Clinical Case of Post-Vaccination Measles Followed By Severe Neutropenia
We present a 13 - month old girl, who develop a post vaccination measles infection after a MMR vaccine , followed by a severe neutropenia. The hematological changes last more than one year and resolved spontaneously. We...