Optimization of the treatment and extraction procedures for Cetellaasiatica L. Urban.
Journal Title: International Journal of Pharmaceutical Science Invention - Year 2017, Vol 6, Issue 6
Abstract
The study is to research and apply enzyme Viscozyme supported for increase the possibility of constituents from Centellaasiatica (CA) that has biological effectiveness. The results show that the Centellaasiatica extract (CAE) yield is higher twice when comparing with the classical extractmethod (only using ethanol solvent). The detailed results: Surveying pre-treatment conditions of Viscozyme reached to the highest effectiveness at the concentration 2%, at 450C, in 90 minutes with Centella/ enzyme: 1/8. After CA treated with by enzyme will be extracted twice with ethanol 80%, in 60 minutes and at 400C with Centella/enzyme: 1/9 (w/v) whichreached 46,22%. The optimization conducted RSM-CCD Minitab 16 software with the results: CA treated withViscozyme 2,5%, at 430C. Then, extracted three times by ethanol 75% with mixture ratio ratio of solid to solvent (1/9 w/v), the optimized CAE yield is 49,21%, The method of optimization is used Minitab 16 that helped to reducethe extracted ethanol concentration(5%), temperature at 50C, also reducing the extracted time concentration (5,5 hours) when comparing with the old method. The extraction of asiaticoside from optimized CAE process (RMT) reached 37,91 mg/ml, higher 2,32 times when comparing with the classical extract method (RM1) only reached 16,31 mg/ml. The study has applied enzyme in the process which helped the time of the extracted process shorter, reducing temperature and the concentration of the extracted solvent, increasing the content of extracted asiaticoside.
Authors and Affiliations
Nguyen Thai Binh, Huynh Ngoc Oanh
Keywords
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